Pierce™ Protease and Phosphatase Inhibitor Mini Tablets

Catalog number: 88668

Thermo Scientific™  Related applications: Protein Purification & Isolation

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Description

Thermo Scientific Pierce Protease and Phosphatase Inhibitor Tablets contain both protease inhibitors and phosphatase inhibitors to provide broad-spectrum complete protection from dephosphorylation and proteolytic degradation.

Features of Protease and Phosphatase Inhibitor Tablets:

Complete—all-in-one tablet contains both protease and phosphatase inhibitors
Easy-to-use—the refrigerator-stable tablets are easily dissolved in buffers and lysis reagents
Compatible—use with homebrew or commercially available cell lysis reagents and Thermo Scientific Protein Assays

The Pierce Protease and Phosphatase Inhibitor Tablets contain aprotinin, bestatin, E-64, EDTA, and leupeptin to provide protection from degradation by serine-proteases, cysteine-proteases, aspartic acid-proteases. To prevent the dephosphorylation activity of serine/threonine and tyrosine phosphatases, each tablet also contains sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. This formulation contains EDTA, a metalloproteinase inhibitor that may interfere with certain downstream applications. Pierce Protease and Phosphatase Inhibitor Tablets are stable at 4°C for up to 12 months, and each tablet is sufficient for 10 mL of solution.

Applications:
• Protect intact, active cellular proteins from degradation and/or dephosphorylation by endogenous proteases and phosphatases.
• Preserve protein integrity and phosphorylation upstream of phosphoproteomic analyses and phospho-specific immunoassays.

Pierce Protease and Phosphatase Inhibitor Tablets are the first complete inhibitor tablet. Adding one tablet to 10 mL of lysis buffer provides complete protection from protease and phosphatase activity during protein extraction and protein purification. There are two formulations of this inhibitor available, one containing EDTA and one without. While both formulations have metalloprotease inhibitors, an EDTA-free formulation is available for applications that require divalent cations for protein activity. Similarly, EDTA and certain phosphatase inhibitors interfere with immobilized metal chelate affinity chromatography (IMAC) and 2D electrophoresis. Dialyze or desalt the sample before performing these procedures. The Protease and Phosphatase Inhibitor Tablets formulation is mass spec-compatible because it does not contain AEBSF.

More Product Data
Complete protein protection from proteases and phosphatases

Related Products
Pierce™ Protease and Phosphatase Inhibitor Mini Tablets, EDTA Free
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Phosphatase Inhibitor, Protease Inhibitor
Form: Tablets
Product Size: 20 tablets

Contents & storage

Upon receipt store at 4°C. Product shipped with ice packs. Note: Tablets may discolor if stored for extended periods at room temperature.

Documents

Manuals & protocols

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