Pierce™ Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit

Catalog number: 88811

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific Pierce Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit is for isolating phosphopeptides from complex biological samples using titanium dioxide-coated magnetic beads.

Features of the Magnetic Titanium Dioxide Phosphopeptide Enrichment Kit:

Complete MS-compatible kit—includes ready-to-use binding, wash and elution buffers that are optimized for phosphopeptide enrichment and downstream analysis by MALDI and ESI mass spectrometry
Optimized for HTS—procedure validated for processing 1 to 96 samples at a time; complete entire assay in about 15 minutes using a KingFisher Flex Instrument
Stable affinity ligand—titanium dioxide is specially coated as a film on the magnetic particles
Selective—affinity system is selective for phosphorylated Ser, Tyr and Thr; exhibits minimal non-specific binding to acidic residues
Sensitive—affinity provides more than 1000 times greater sensitivity than traditional IMAC technologies; enables enrichment and MS-measurement of less than 100 fmol of phosphoprotein

The TiO2 ligand selectively binds peptides containing phosphorylated serine (Ser), tyrosine (Tyr) or threonine (Thr), enabling phosphopeptide enrichment from protease-digested samples. The isolated phosphopeptides are compatible for analysis downstream by mass spectrometry. The high-performance, iron oxide, superparamagnetic particles are validated and optimized for use with high-throughput magnetic platforms, such as the Thermo Scientific KingFisher 96 and KingFisher Flex Instruments. The beads also enable premium performance for simple benchtop applications using an appropriate magnetic stand.

More Product Data
Quantitative cancer stem cell phosphoprotein profiling
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 96 reactions
Final Product: Peptide
Workflow Step: Sample Enrichment
Detection Method: Mass Spectrometry
Starting Material: Protease-digested protein, Peptides
For Use With (Equipment): Mass Spectrometer, Microcentrifuge

Contents & storage

Upon receipt store at 4°C. Product shipped on an ice pack.

Documents

Manuals & protocols

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