pT7CFE1-CMyc Vector for Mammalian Cell-Free Protein Expression

Catalog number: 88864

Thermo Scientific™  Related applications: Protein Expression

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Description

Thermo Scientific pT7CFE1-CMyc is a cloning plasmid optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins. pT7CFE1-CMyc Vector is available with single affinity Myc tag at the C-terminus to facilitate protein purification and detection.

Features of pT7CFE1:
• EMCV IRES at the 5' UTR promotes high-level translation of mRNAs
• MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
• Poly A sequence in the 3' region promotes mRNA stabilization and protection from nucleases
• T7 terminator ensures synthesis of accurate size mRNA transcripts
• Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region

pT7CFE1 Expression Vectors contain the Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) element that is critical for high levels of cap-independent protein expression in the Human In Vitro Translation System. Each vector features a highly-compatible multiple cloning site (MCS) to facilitate easy insertion of protein coding sequences into and between vectors. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE Vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation. Custom cloning services are also available.

More Product Data
Choosing a vector and purification method for in vitro protein expression

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For Research Use Only. Not for use in diagnostic procedures.

Specifications

Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 10 µg
Promoter: T7
Protein Tag or Fusion: c-Myc Epitope Tag

Contents & storage

Store between -70°C and -20°C.

Documents

Manuals & protocols