Thermo Scientific™

pT7CFE1-NFtag Vector for Mammalian Cell-Free Protein Expression

Catalog number: 88865
Thermo Scientific™

pT7CFE1-NFtag Vector for Mammalian Cell-Free Protein Expression

Catalog number: 88865
Catalog Number
88865
Unit Size
10 µg
Price (USD)
Price: 110.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
8886510 µg
Price: 110.00
Your Price:
***
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Thermo Scientific pT7CFE1-NFtag is a cloning plasmid optimized to use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System for in vitro translation (IVT) of tagged fusion proteins. pT7CFE1-NFtag Vector is available with single affinity Ftag tag at the N-terminus to facilitate protein purification and detection.

Features of pT7CFE1:
• EMCV IRES at the 5' UTR promotes high-level translation of mRNAs
• MCS accommodates gene insertion via ten different restriction sites: Msc1, Nde1, BamH1, EcoR1, EcoRV, Pac1, Pst1, Sac1, Sal1, Not1 and Xho1
• Poly A sequence in the 3' region promotes mRNA stabilization and protection from nucleases
• T7 terminator ensures synthesis of accurate size mRNA transcripts
• Plasmid linearization may be accomplished with restriction sites between Poly A sequence and the T7 terminator region

pT7CFE1 Expression Vectors contain the Encephalomyocarditis virus (EMCV) internal ribosome entry site (IRES) element that is critical for high levels of cap-independent protein expression in the Human In Vitro Translation System. Each vector features a highly-compatible multiple cloning site (MCS) to facilitate easy insertion of protein coding sequences into and between vectors. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection. The pT7CFE Vectors are suitable for insertion of cloned genes, cDNAs, ORFs or PCR products for in vitro transcription and translation. Custom cloning services are also available.

More Product Data
Choosing a vector and purification method for in vitro protein expression

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pT7CFE1-NHis-GST-CHA Vector for Mammalian Cell-Free Protein Expression
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Description
pT7CFE1-NFtag Vector
Type
pT7CFE1-NFtag Vector
Cloning Method
Restriction Enzyme/MCS
Protein Tag
FLAG
Quantity
10 µg
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Promoter
T7

Contents & Storage

Store between -70°C and -20°C.

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