The price per kit will be increasing on September 1, 2017. This product will be unavailable for purchase after December 31, 2017. We recommend Cat. No. 88890 as an alternative. Learn about our cell free expression systems. Please contact us with any questions. Custom orders will still be available for qualifying purchases with set minimum purchase quantities, please contact us for more information.

1-Step CHO High-Yield IVT Kit

Catalog number:  88893

Thermo Scientific™  Related applications: Protein Expression

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Description

The Thermo Scientific™ 1-Step CHO High-Yield In Vitro Translation (IVT) Kit enables the expression of functional proteins with up to 750 times greater yield per mL of reaction than rabbit reticulocyte lysate-based mammalian IVT systems that only yield up to 10 μg protein/mL reaction.

• High expression—up to 750 µg/mL of expressed protein
• Reproducible results—low variability between experiments
• Fast—express high levels of protein with 6 hours to overnight incubation
• Easier storage—allows you to store lyophilized CHO cell lysate at -20°C
• Functional—obtain functionally active proteins, including those containing disulfide bonds
• Easy-to-use—three reaction components together carry out transcription and translation in one step
• Great for labeling—amenable to incorporation of labeled and unnatural amino acids
• Vector compatibility—uses existing pT7CFE1 vectors for protein expression

The 1-Step CHO High-Yield IVT System uses extracts containing the robust translation machinery of the pharmaceutical industry standard Chinese Hamster Ovary (CHO) cell line to generate functional full-length proteins. In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids, and energy-generating substrates into the reaction while removing inhibitors of proteins synthesis. This continuous-exchange cell-free (CECF) system enables protein expression in an overnight incubation of up to 750 µg/mL. The CHO IVT kit includes all of the components required for transcription and translation of a recombinant gene, including an optimized expression vector.

Includes:
Lyophilized cell lysate, reaction mix, accessory proteins, 4X dialysis buffer, control DNA, cloning vector, dialysis devices, and centrifuge tubes.

Requires:
30°C incubator or water bath (shaker incubator increases expression by 30 to 50%)

Applications:
• Production of large amounts of recombinant protein for structural analysis
• Generation of active proteins for downstream applications such as protein interaction studies
• Rapid large scale expression and characterization of mutant proteins
• Production of viral particles
• Incorporation of unnatural amino acids
• Production of cytotoxic proteins
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Expression: Cell-Free
Product Size: 2 x 100 µL

Contents & storage

Lyophilized CHO lysate, 1 vial
Accessory proteins, 25 μL
Dialysis buffer (4X), 900 μL
Reaction mix, 40 μL
Positive control DNA (pCFE-GFP), 10 μg
pT7CFE1-NHis-GST-CHA, 10 μg
Microdialysis device, 2 each
Nuclease-free water, 50 mL

Store in ultra-cold freezer (-68 to -85°C).
Sufficient for 2 reactions of 100 μL each.

Documents

Manuals & protocols

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