Thermo Scientific™


Catalog number:  88900

Related applications:

Protein Labeling & Crosslinking

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Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

Features of NHS-Phosphine:

Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Related Products
For Research Use Only. Not for use in diagnostic procedures.


Product Line: Pierce™
Crosslinker Type: Heterobifunctional
Labeling Method: Chemical Labeling
Reactive Moiety: NHS Ester, Phosphine
Solubility: DMSO (Dimethylsulfoxide), DMF (Dimethylformamide)
Spacer: Short
Description: Pierce NHS-Phosphine

Contents & storage

Upon receipt store at -20°C protected from light and moisture. Product is shipped at ambient temperature.