NHS-Phosphine
NHS-Phosphine
Thermo Scientific™

NHS-Phosphine

Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymerRead more
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Catalog number 88900
Price (USD)/ Each
459.00
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Quantity:
10 mg
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Price (USD)/ Each
459.00
Add to cart
NHS-Phosphine
Catalog number88900
Price (USD)/ Each
459.00
-
Add to cart
Thermo Scientific NHS-Phosphine is an amine-reactive compound that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to azide-tagged molecules.

Features of NHS-Phosphine:

Soluble—reagent easily dissolves in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Specific—NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)
Chemoselective—azide and phosphine groups do not react or interfere with components of biological samples (bioorthogonal) but conjugate to one another with high efficiency

This NHS-ester compound reacts to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The phosphine group (PPh2) reacts with azide-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different azide-tagged compounds, this NHS-Phosphine reagent facilitates a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.

Related Products
NHS-Phosphine
NHS-Azide
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine-Azide
CleavableNo
DescriptionNHS-Phosphine
Molecular Weight (g/mol)461.4
PEGylatedNo
Spacer Arm Length5.4 Å
Cell PermeabilityNot determined
Shipping ConditionAmbient
Product LinePierce™
Labeling MethodChemical Labeling
Crosslinker TypeHeterobifunctional
Reactive MoietyNHS Ester, Phosphine
SpacerShort (<10 Å)
FormPowder
Quantity10 mg
SolubilityDMF, DMSO
FormatStandard
Water SolubleNo
Product TypeCrosslinker
Unit SizeEach
Contents & Storage
Upon receipt store at -20°C protected from light and moisture.