Thermo Scientific™

EZ-Link™ Phosphine-PEG3-Biotin

Catalog number:  88901

Related applications:

Protein Biology

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Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

Features of EZ-Link Phosphine-PEG3-Biotin:

Soluble—easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Chemoselective—the phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that biotinylation is specific
PEG spacer—polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein

When used in combination with azide labeling strategies, this compound enables detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins. The phosphine group of Phosphine-PEG3-Biotin conjugates to azide groups by the Staudinger reaction mechanism. Azide groups can be introduced into proteins or other cellular targets through in vivo labeling with azide-tagged derivatives of naturally occurring metabolic building blocks. Because neither phosphines nor azides are present in biological systems, they comprise a chemoselective (mutually specific) ligation pair for labeling and conjugation.
For Research Use Only. Not for use in diagnostic procedures.


Product Line: EZ-Link™
Cell Permeability: Cell-Impermeant
Chemical Reactivity: Azide
Label Type: Biotin & Analogs
Label or Dye: Biotin
Reactive Moiety: Phosphine
Solubility: DMSO (Dimethylsulfoxide), Water, DMF (Dimethylformamide)
Spacer: Extra-Long, Pegylated

Contents & storage

Store at -20°C. Shipped at ambient temperature.