Thermo Scientific™

EZ-Link™ Phosphine-PEG3-Biotin

Catalog number: 88901
Thermo Scientific™

EZ-Link™ Phosphine-PEG3-Biotin

Catalog number: 88901
Catalog Number
88901
Unit Size
10 mg
Price (USD)
Price: 626.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
8890110 mg
Price: 626.00
Your price:
-
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Thermo Scientific Pierce EZ-Link Phosphine-PEG3-Biotin is a biotinylation reagent for labeling azide-containing molecules, which enables biotin-based detection and affinity purification of molecules via Staudinger ligation strategies.

Features of EZ-Link Phosphine-PEG3-Biotin:

Soluble—easily dissolves in water-miscible solvents (e.g., DMSO) for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples
Compatible—reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents
Chemoselective—the phosphine reactive group is specific in biological samples for bioorthogonal azide-tagged molecules, ensuring that biotinylation is specific
PEG spacer—polyethylene glycol spacer arm helps maintain solubility of labeled molecules and decreases steric hindrance for affinity-binding to avidin, streptavidin or NeutrAvidin Protein

When used in combination with azide labeling strategies, this compound enables detection or affinity purification of protein interactions and post-translational modifications using streptavidin probes or streptavidin agarose resins. The phosphine group of Phosphine-PEG3-Biotin conjugates to azide groups by the Staudinger reaction mechanism. Azide groups can be introduced into proteins or other cellular targets through in vivo labeling with azide-tagged derivatives of naturally occurring metabolic building blocks. Because neither phosphines nor azides are present in biological systems, they comprise a chemoselective (mutually specific) ligation pair for labeling and conjugation.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Phosphine-PEG3-Biotin
Quantity
10 mg
Product Line
EZ-Link™
Cell Permeability
Cell-Impermeant
Chemical Reactivity
Azide
Reactive Moiety
Phosphine
Solubility
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
Label or Dye
Biotin
Spacer
Extra-long, Pegylated
Label Type
Biotin & Analogs

Contents & Storage

Store at -20°C. Shipped at ambient temperature.

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