Pierce™ LDH Cytotoxicity Assay Kit

Pierce™ LDH Cytotoxicity Assay Kit - FAQs

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3 product FAQs found

What could cause my absorbance values to be low when reading my plate after performing the LDH assay?

This could be due to low cell density. We recommend performing a serial dilution of your cells to determine appropriate cell density for the LDH Cytotoxicity Assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do you recommend using the minimal amount of serum in the media when growing my cells for performimng an LDH cytotoxicity assay?

Serum will contribute some inherent LDH activity which will elevate background levels. Keeping the amount of serum low will reduce the background. We recommend researchers perform preliminary studes to ensure that the percent serum included in their samples is sufficient to keep their cells healthy and alive.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do I have to read my samples at both 490 nm and 680 nm when using the LDH Cytotoxicity Assay Kit?

Reading the samples at 680 nm and subtracting this value from the absorbance at 490 nm will eliminate background signal from the instrument.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.