Chemiluminescent Nucleic Acid Detection Module Kit

Catalog number: 89880

Thermo Scientific™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Description

The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional radioactive (32P) systems. The module consists of the essential detection components used in the Thermo Scientific North2South Kit and Thermo Scientific LightShift EMSA Kit. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that provide for fast, trouble-free detection on film or by CCD camera. Obtain publication-quality blots having excellent signal and low background.

Features of the Chemiluminescent Nucleic Acid Detection Module:

Fast and sensitive—achieve the same speed and detection levels you've come to expect with Thermo Scientific North2South and LightShift Kits
Versatile—kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
Flexible—kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Related Products
Nucleic Acid Detection Blocking Buffer
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Size: 10 blots

Contents & storage

Sufficient For: 800 cm2 of membrane (10 mini-gel blots)
• Stabilized Streptavidin-HRP, 1.5 mL
• Luminol/Enhancer Solution, 80 mL
• Stable Peroxide Solution, 80 mL
• Blocking Buffer (Part No. 89880A), 500 mL
• Wash Buffer (4X), 500 mL
• Substrate Equilibration Buffer, 500 mL

Store at 4°C.

Documents

Manuals & protocols