In-Solution Tryptic Digestion and Guanidination Kit

Catalog number: 89895

Thermo Scientific™  Related applications: Protein Mass Spectrometry Analysis

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Description

The Thermo Scientific Pierce In-Solution Tryptic Digestion and Guanidination Kit contains proteomics-grade trypsin, buffers and reagents to alkylate and digest proteins and then guanidinate the peptide fragments for mass spectrometry.

Features of the kit:

Optimized trypsin digestion—produce complete digests of protein ranging from 0.025 to 10 µg
Optimized guanidination—reaction produces little to no N-terminal modification
Convenient—kit includes reagents for digestion, reduction, alkylation and guanidination.
Quick—protein can be digested and guanidinated all in one day

Accurate identification of proteins and analysis of post-translational modifications by mass spectrometry require accurate and complete protein digestion and peptide modification. The In-Solution Tryptic Digestion and Guanidination kit provides an optimized procedure and reagents for approximately 90 digests, each containing 0.025 to 10 µg of protein.

Trypsin specifically cleaves peptide bonds at the carboxyl side of arginine and lysine residues. The signals from the arginine-containing peptides are generally stronger, due to the more basic side chain. To enhance overall ionization, guanidination is necessary to convert lysines to homoarginines. The guanidination reaction is specific for the amine of lysine but may occur minimally at the amine of the peptide's N-terminus. This derivatization leads to an increase in the intensity of the lysine containing peptides and improved sequence coverage overall.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer
Product Size: 90 reactions
Starting Material: Cell Lysate, Protein samples
Workflow Step: Protein Digestion

Contents & storage

Upon receipt store the Pierce Trypsin Protease at -20°C in a nonfrost-free freezer; store other components at 4°C. Kit is shipped on gel pack.

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