Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.
Features of Sodium Deoxycholate:
• Popular anionic, bile-acid detergent for many laboratory uses • Effective in disrupting and dissociating many types of protein interaction • Can be removed from solution by dialysis • Useful for elution or regeneration of certain kinds of affinity columns • High-purity compound with low UV absorptivity
Properties of Sodium Deoxycholate: • Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt • Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt • Molecular Weight: 414.6g • Detergent Class: Ionic (anionic) • Aggregation Number: 5 (average) • Micelle Molecular Weight: 2000g (average) • Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v) • Cloud Point: Unknown • Dialyzable: Yes
Specifications for Sodium Deoxycholate (Part No. 89904, 89905): • Formula: C24H39O4Na • Molecular Weight: 414.6g • Purity (by HPLC): ≥98% • Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06 • pH (1% Solution): 5 to 9 • Solubility (in water at 20°C): ≥5%
Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.
The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ∼2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.
Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.