Thermo Scientific™

Pierce™ Phosphoprotein Enrichment Kit

Catalog number: 90003
Thermo Scientific™

Pierce™ Phosphoprotein Enrichment Kit

Catalog number: 90003
Catalog Number
90003
Unit Size
10 columns
Price (USD)
628.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
9000310 columns
628.00
Your Price:
***
Product Overview
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The Thermo Scientific Pierce Phosphoprotein Enrichment Kit is designed for the efficient enrichment of phosphorylated proteins derived from mammalian cells and tissues.

Features of the Phosphoprotein Enrichment Kit:

Specific - low nonspecific protein contamination from complex biological samples, such as cell culture lysate and mouse tissue extract
Fast - easy spin format enables enrichment of phosphorylated proteins in less than 2 hours
Superior yield - acheives higher yields than other commercially available kits
Convenient format - complete kit offering pre-dispensed spin columns, buffers, reagents and ultrafiltration columns for enrichment
Compatible - downstream applications include mass spectrometry, western blotting, and 2D-PAGE

The proprietary resin and buffer composition produces superior yields with negligible non-specific binding. The kit is uses a convenient spin column format and is compatible with downstream applications including Western blotting, Mass Spec, 2D-PAGE, and protein arrays. The affinity resin is also available as pre-packed chromatography cartridges for use with automated liquid chromatography (LC) systems.

Phosphorylation is one of the most frequently occurring post-translational modifications in proteins and it regulates nearly all intracellular biological events including, signal transduction, protein-protein interaction, protein stability, protein localization, apoptosis, and cell cycle control. Detecting changes in protein phosphorylation can be a difficult task, because of the transient labile state of the phosphate group. Furthermore, low phosphoprotein abundance and poorly developed phospho-specific antibodies also contribute to difficulties in phosphoprotein detection. Recent advances in mass spectrometry (MS) technology in combination with phosphoprotein enrichment using immobilized metal affinity chromatography (IMAC) have resulted in greater resolution of the phosphoproteome.

More Product Data
Phosphoprotein enrichment from cell and tissue samples
Quantitative cancer stem cell phosphoprotein profiling

Related Products
Pierce™ Phosphoprotein Enrichment Chromatography Cartridges, 5 mL
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity
10 Column Kit
Stationary Phase
Proprietary Ligand
Sufficient For
10 Reactions
Column Type
Affinity, Agarose Resin
Form
50 mL Conical, Kit, Spin Column
Product Line
Pierce™
Target
Phosphoproteins
Type
Phosphoprotein Enrichment Kit

Contents & Storage

Upon receipt store at 4°C.

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