Thermo Scientific™

Pierce™ Lys-C Protease, MS Grade

Catalog number:  90051

 Related applications:

Protein Mass Spectrometry Analysis

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Thermo Scientific Lys-C Protease, MS Grade, is a highly purified native endoproteinase validated for maximum activity and stability in proteomic applications.

Features of MS Grade Lys-C Protease:

• Enhanced digestion—use in tandem with trypsin to decrease tryptic missed cleavages
• Increased sequence coverage—better protein characterization results from overlapping peptides with complementary chromatographic, ionization and fragmentation properties
• Versatile—effective enzyme activity under highly denaturing conditions (e.g., 8M urea)
• C-terminal lysine cleavage specificity—at least 90% for a complex protein sample
• Stable—provided in a lyophilized format

Product Details
This Lys-C is a mass spectrometry (MS)-grade serine protease isolated from Lysobacter enzymogenes. Lys-C has high activity and specificity for lysine residues resulting in larger peptides and less sample complexity than trypsin (i.e. fewer peptides). Unlike trypsin, Lys-C can cleave lysines followed by prolines, making it ideal for sequential protein digestion followed by trypsin to decrease missed cleavages. These unique Lys-C properties ensure high digestion efficiency when used alone or followed by tryptic digestion. Additionally, Lys-C prototypic peptides typically have higher charge states, making it an enzyme of choice for use with ETD fragmentation. Lys-C is commonly in phosphopeptide enrichment workflows because it generates peptides with primary amines at both the N-and C-terminus allowing the fragments to be double-labeled with amine-reactive isobaric tags. This results in enhanced peptide ionization and improved limits of quantitation since more fragment ions can be re-isolated during MS3 acquisition. This enzyme can be used for in-solution or in-gel digestion workflows to produce peptides for LC-MS/MS protein identification. This Lys-C enzyme is packaged lyophilized (20 µg or 100 µg quantities).

The endoproteinase LysC specifically hydrolyzes proteins at the carboxyl side of lysine. Efficient protein digestion can be completed in 2 hours at 37°C. LysC remains active in highly denaturing conditions such as 8M urea, 2M guanidine·HCl, 1% SDS, 2% CHAPS and 40% acetonitrile and functions well within pH 7-9 (maximal activity at pH 8). This lyophilized enzyme has a mass of 30 kDa and is stable for 1 year when stored at -20°C.

• Improved sequence coverage of protein digests
De novo sequencing
• Epigenetic studies
• In-gel and in-solution digestion of proteins
For Research Use Only. Not for use in diagnostic procedures.


Bulk or Custom Form: Bulk Custom Form 1
Detection Method: Mass Spectrometry
Final Product: Peptide
For Use With (Equipment): Mass Spectrometer
Product Line: Pierce™
Product Size: 20 µg
Starting Material: Cell Lysate, Protein samples
Workflow Step: Protein Digestion

Contents & storage

Store at -20°C in a nonfrost-free freezer.


Manuals & protocols