Tandem Mass Tag™ 2-plex (TMTduplex™) Isotopic Label Reagents comprise “light” TMTzero and “heavy” TMT6-127 label reagents while Tandem Mass Tag™ 2-plex (TMTduplex) Isobaric Label Reagents comprise “heavy” TMT2-126 and TMT2-127 Label Reagents for multiplexing. Both sets of label reagents have the same structure that are NHS-activated for covalent, irreversible labeling of primary amine groups (—NH2).

Tandem Mass Tag (TMT) Reagents are chemical tags that enable identification and quantitation of proteins in different samples using tandem mass spectrometry. The amine-reactive, NHS-ester-activated compounds covalently attach to the peptide amino terminus and free amino termini of lysine residues of peptides and proteins with high efficiency, thereby labeling all peptides in each sample regardless of enzyme used for digestion.

During MS/MS analysis, each TMT tag is also fragmented to produce a unique reporter ion mass that is used for sample identification and quantitation. Protein quantitation is accomplished by comparing the relative intensities of the two reporter ions in the MS/MS spectra. Our TMTduplex reagents label all peptides for analysis of two samples in a single MS analysis.

Features of TMTduplex Isobaric and Isotopic Label Reagent Sets:
•Powerful—concurrent MS analysis of two samples increases throughput and enables relative quantitation of two different treatment conditions (up to 5 experiments per set)
•Consistent—identical reagent structure and performance among TMTzero, TMTduplex, TMTsixplex and TMT10plex reagents allows efficient transition from method development to multiplex quantitation, enabling biomarker discovery research across platforms and data sets
•Robust—consistent chemistry allows efficient transition from method development to multiplex quantitation
•Efficient—amine-reactive NHS-ester activated reagents ensure efficient labeling of all peptides regardless of protein sequence or proteolytic enzyme specificity
•Compatible—optimized for use with high resolution Thermo Scientific Orbitrap MS/MS platforms, with data analysis fully supported by Proteome Discoverer™ 2.4 and above

Applications:
• Protein expression profiling of normal vs. disease states or control vs. treated
• Measurement of two different samples concurrently in a single experiment
• Quantitative analysis of proteins for which no antibodies are available
• dentification and quantitation of membrane and post-translationally modified proteins
• Identification and quantitation of hundreds to thousands of proteins in a single experiment

TMT Reagents are provided as standalone sets or in optimized kit formats containing all necessary reagents and controls for maximum flexibility, convenience and reliability. Tandem Mass Tag Reagents combined with the industry leading instruments and software provide integrated total system solutions for quantitative protein expression analysis.