Thermo Scientific™

TMT™ Elution Buffer

Catalog number: 90104
Thermo Scientific™

TMT™ Elution Buffer

Catalog number: 90104
Catalog Number
90104
Unit Size
20 mL
Price (USD)
80.00
Your Price:
Availability
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Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
9010420 mL
80.00
Your Price:
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Product Overview
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Thermo Scientific TMT Elution Buffer is for use with Thermo Scientific Immobilized Anti-TMT Resin to enrich samples for peptides that have been chemically labeled with Tandem Mass Tag™ (TMT™) Reagents.

Immobilized Anti-TMT Resin and TMT Elution Buffer are effective for reducing sample complexity, improving dynamic range and studying cysteine modifications such as S-nitrosylation, oxidation and disulfide bonds of protein samples labeled with iodoTMT Reagents. This approach of selective thiol labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for higher multiplex quantitation.

The Immobilized Anti-TMT Resin uses the highly specific Anti-TMT Antibody to bind peptides labeled with TMT Reagents. The antibody is specific for the mass reporter region of the TMT Reagents which allows for enrichment of TMT-labeled peptides regardless of peptide labeling chemistry (e.g., amine-reactive, sulfhydryl-reactive). The TMT Elution Buffer is a volatile neutral buffer which competitively elutes captured TMT-labeled peptides. When used in combination with the Immobilized Anti-TMT Resin, the TMT Elution Buffer provides higher specific enrichment of TMT-labeled peptides compared to acidic or basic elution buffers.

Related Products
Immobilized Anti-TMT™ Antibody Resin
iodoTMTsixplex™ Label Reagent Set, 1 x 0.2 mg
iodoTMTsixplex™ Isobaric Mass Tag Labeling Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Method
Mass Spectrometry
Final Product Type
Peptide
For Use With (Equipment)
Mass Spectrometer
Label Type
Tandem Mass Tag™ (TMT) Labels
Product Line
TMT™
Starting Material
Cell Lysate
Workflow Step
Protein Labeling

Contents & Storage

Store at 4°C.

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