Immobilized Anti-TMT Resin and TMT Elution Buffer are effective for reducing sample complexity, improving dynamic range and studying cysteine modifications such as S-nitrosylation, oxidation and disulfide bonds of protein samples labeled with iodoTMT Reagents. This approach of selective thiol labeling and affinity enrichment is similar to isotope-coded affinity tags (ICAT™ Method) but allows for higher multiplex quantitation.
The Immobilized Anti-TMT Resin uses the highly specific Anti-TMT Antibody to bind peptides labeled with TMT Reagents. The antibody is specific for the mass reporter region of the TMT Reagents which allows for enrichment of TMT-labeled peptides regardless of peptide labeling chemistry (e.g., amine-reactive, sulfhydryl-reactive). The TMT Elution Buffer is a volatile neutral buffer which competitively elutes captured TMT-labeled peptides. When used in combination with the Immobilized Anti-TMT Resin, the TMT Elution Buffer provides higher specific enrichment of TMT-labeled peptides compared to acidic or basic elution buffers.