Thermo Scientific™

TMT™ Elution Buffer and Anti-TMT Resin

Catalog number: 90104
Thermo Scientific™

TMT™ Elution Buffer and Anti-TMT Resin

Catalog number: 90104
Tandem mass tag (TMT) isobaric label reagents are used for multiplexed protein quantitation using tandem mass spectrometry. Immobilized Anti-TMT Resin is designed to effectively capture and enrich peptides or proteins labeled using any TMT reagent. Anti-TMT Resin and TMT Elution Buffer are effective for reducing sample complexity, improving dynamic range, and studying cysteine modifications such as S-nitrosylation, oxidation, and disulfide bonds of protein samples labeled with iodoTMT reagents.
 
Catalog Number
90104
Unit Size
20 mL
Description
TMT™ Elution Buffer
Quantity
20 mL
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionTMT™ Elution Buffer
For Use With (Equipment)Mass Spectrometer
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Final Product TypePeptide
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity20 mL
Unit Size20 mL
Catalog Number
Unit Size
6 mL
Description
Immobilized Anti-TMT™ Antibody Resin
Quantity
6 mL
Price (USD)
Availability
-
Full specifications
Quantity
DescriptionImmobilized Anti-TMT™ Antibody Resin
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity6 mL
Unit Size6 mL
Showing 2 of 2
Catalog NumberSpecificationsUnit SizeDescriptionQuantityPrice (USD)AvailabilityQuantity
90104Full specifications
20 mLTMT™ Elution Buffer20 mL
-
DescriptionTMT™ Elution Buffer
For Use With (Equipment)Mass Spectrometer
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Final Product TypePeptide
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity20 mL
Unit Size20 mL
90076Full specifications
6 mLImmobilized Anti-TMT™ Antibody Resin6 mL
-
DescriptionImmobilized Anti-TMT™ Antibody Resin
Product LineTMT™
Label or DyeTandem Mass Tag™ (TMT) Labels
Starting MaterialCell Lysate
Detection MethodMass Spectrometry
Workflow StepProtein Labeling
Quantity6 mL
Unit Size6 mL
Showing 2 of 2

Tandem mass tag (TMT) reagents are used to chemically label proteins in different samples for multiplexed quantitation using tandem mass spectrometry. Immobilized Anti-TMT Resin contains an agarose-immobilized anti-TMT monoclonal antibody and is effective for capture and enrichment of peptides or proteins labeled using any TMT reagent. This helps simplify otherwise complex samples before LC-MS/MS analysis, especially when the targets for labeling are rare (e.g., cysteine sulfhydryls). Enrichment of labeled peptides by this method results in fewer peptides identified per protein, but can significantly increase the number of unique proteins identified, making it easier to detect and measure low-abundance proteins.

Immobilized Anti-TMT Resin uses our highly specific Anti-TMT Antibody (Cat. No. 90075) to bind peptides labeled with TMT reagents. The antibody is specific for the mass reporter region of the TMT reagents, which allows for enrichment of TMT-labeled peptides regardless of peptide labeling chemistry (e.g., amine-reactive, sulfhydryl-reactive). TMT Elution Buffer is a volatile neutral buffer that competitively elutes captured TMT-labeled peptides. When used in combination with Immobilized Anti-TMT Resin, TMT Elution Buffer provides higher specific enrichment of TMT-labeled peptides compared to acidic or basic elution buffers.

Enrichment features include:
Specific—uses specific, high-affinity antibody for the TMT structure (Kd >100 pM)
Compatible—effective for all varieties of TMT reagents (amine-reactive or cysteine-reactive)
Versatile—use resin with TMT Elution Buffer for optimal sample enrichment

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store at 4°C.

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