The GeneChip™ HT WT PLUS Reagent Kit (HT WT PLUS Kit) is designed for automation of the target preparation process for expression analysis on whole-transcriptome (WT) arrays. Designed with reagent chemistries identical to GeneChip WT PLUS Reagent Kit, this high-throughput (HT) solution allows the user to generate hybridization-ready targets from input RNA with minimal hands-on time.
HT WT PLUS Kit uses a reverse transcription priming method that primes the entire length of each RNA transcript, including both poly-A and non-poly-A mRNA to provide complete and unbiased coverage of the transcriptome. The kit efficiently generates amplified and biotinylated sense-stranded DNA targets, avoiding loss of specificity due to antisense strand interference.
Features and benefits • A complete target prep kit with proven sensitivity and specificity due to an optimized chemistry for WT arrays • Automated protocol that enables the processing of up to 96 samples with minimal hands-on time • Ability to run a variety of sample types, including clinically relevant samples such as whole blood, to enable robust biomarker identification • Low sample input requirement (50 ng), allowing you to save your precious samples for further downstream studies • No requirement for ribosomal RNA depletion or a globin reduction step for blood samples