Applied Biosystems™

GeneChip™ HT WT PLUS Reagent Kit

Catalog number:  902414

Related applications:

Microarray Analysis

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Description

The GeneChip™ HT WT PLUS Reagent Kit (HT WT PLUS Kit) is designed for automation of the target preparation process for expression analysis on whole-transcriptome (WT) arrays. Designed with reagent chemistries identical to GeneChip WT PLUS Reagent Kit, this high-throughput (HT) solution allows the user to generate hybridization-ready targets from input RNA with minimal hands-on time.

HT WT PLUS Kit uses a reverse transcription priming method that primes the entire length of each RNA transcript, including both poly-A and non-poly-A mRNA to provide complete and unbiased coverage of the transcriptome. The kit efficiently generates amplified and biotinylated sense-stranded DNA targets, avoiding loss of specificity due to antisense strand interference.

Features and benefits
• A complete target prep kit with proven sensitivity and specificity due to an optimized chemistry for WT arrays
• Automated protocol that enables the processing of up to 96 samples with minimal hands-on time
• Ability to run a variety of sample types, including clinically relevant samples such as whole blood, to enable robust biomarker identification
• Low sample input requirement (50 ng), allowing you to save your precious samples for further downstream studies
• No requirement for ribosomal RNA depletion or a globin reduction step for blood samples

Related Links
HT WT PLUS Consumables for Biomek™ FXP Target Prep Express
GeneChip™ WT PLUS Reagent Kit
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application): Transcriptome Profiling
Product Line: GeneChip™
Product Size: 1 kit
Reagent Type: Sample preparation reagents

Contents & storage

The kit is a complete target preparation solution that provides:
• WT Amplification Kit (Module 1 and Module 2)
• WT Terminal Labeling Kit
• Hybridization Control Kit
• Poly-A RNA Control Kit