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Applied Biosystems™

GeneChip™ Mouse Genome 430 2.0 Assay

The GeneChip™ Mouse Genome 430 2.0 Assay is the first and most comprehensive whole mouse genome expression array:• Complete coverageRead more
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Catalog NumberNumber of Arrays
90249130 arrays
90249010 arrays
Catalog number 902491
Price (INR)
-
Number of Arrays:
30 arrays
The GeneChip™ Mouse Genome 430 2.0 Assay is the first and most comprehensive whole mouse genome expression array:
• Complete coverage of the Mouse Expression Set 430 for analysis of over 39,000 transcripts on a single array
• The Power of the Probe Set - offering multiple independent measurements for each transcript delivering the greatest accuracy and reproducibility of any microarray platform
• More information from every experiment for more complete and efficient analysis

Assay Profile
All probe sets represented on the GeneChip™ Mouse Expression Set 430 are included on the GeneChip Mouse Genome 430 2.0 Assay. The sequences from which these probe sets were derived were selected from GenBank™, dbEST, and RefSeq. The sequence clusters were created from the UniGene database (Build 107, June 2002) and then refined by analysis and comparison with the publicly available draft assembly of the mouse genome from the Whitehead Institute for Genome Research (MGSC, April 2002).

Instrument and Software Requirements
GeneChip Scanner 3000 7G, enabled for High-Resolution Scanning*
GeneChip Operating Software (GCOS) v1.1.1 (includes the GeneChip Scanner 3000 High-Resolution Scanning Patch

*GeneChip Scanner 3000 High-Resolution Update is standard on all instruments shipped starting in September 2003 with serial number series 502. Previous versions, such as serial number series 501, will require the GeneChip Scanner 3000 High-Resolution Update, Catalog Number 00-0110, to be installed.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Microarray Analysis
Product LineGeneChip
Quantity30 reactions
ArrayTranscriptome Profiling
FormatArray Cartridge
Number of Arrays30 arrays
SpeciesMouse
Unit SizeEach

Frequently asked questions (FAQs)

Why were the default gamma values changed for Mouse 430 2.0 Arrays?

The gamma parameters defaults for these arrays were set to deliver specificity consistent with our other expression products. (The changes in the default gamma values apply to 11 micron arrays). These parameters are user definable.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How long does the scan of a Mouse 430 2.0 Array take?

About 12 minutes per Mouse 430 2.0 Array, comparable to scan time for a set of Mouse 430A and Mouse 430B Arrays.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How much memory is needed to store the data from a single Mouse 430 2.0 Array?

A single Mouse 430 2.0 array generates approximately 130MB of data.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Where can I find the high-resolution scanning patch for the Mouse 430 2.0 Arrays?

Please contact Technical Support to obtain the high-resolution scanning patch.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Do I have to apply the high-resolution scanning patch for the Mouse 430 2.0 Arrays?

Below are a list of circumstances and clarification on if the high-resolution scanning patch needs to be applied. Keep in mind that the high-resolution scanning patch must only be applied on top of GCOS, not AGCC software.

a. GeneChip Scanner 3000 (serial numbers 502xxxxx): The instrument service engineer has applied the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at installation, but not to the workstations or servers (see c and d below).

b. GeneChip Scanner 3000 (serial numbers 501xxxxx): The instrument service engineer will apply the high-resolution scanning patch to the computer that is controlling the GeneChip Scanner 3000 at the time of the upgrade, but not to the workstations or servers (see c and d below).

c. Analysis workstations and clients: You must apply the high-resolution scanning patch.

d. Server upgrade: Your database administrator or an Affymetrix Software Support Engineer needs to apply the high-resolution scanning patch in coordination with the upgrade of server to GCOS.

e. Installation of library files post scanner installation or upgrade: The high-resolution scanning patch must be reapplied following the installation of library files for arrays that precede the Mouse 430 2.0 Arrays. The Mouse 430 2.0 and library files are high-resolution-scanning ready. The error message "cannot read the parameters for probe array type from the database" appears when one tries to scan an array on a GeneChip Scanner 3000 high-resolution scanner but the high-resolution scanning patch has not been applied.



Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.