Search

  • Contáctenos
  • Orden Rápida

Clariom™ S Pico Assay, mouse, 30 reactions - FAQs

View additional product information for Clariom™ S Pico Assay, mouse - FAQs (902933)

11 product FAQs found

Is the Clariom S capable of exon-level analysis?

No, the Clariom S array is not capable of exon-level analysis. The probes have selected to be the most constitutive probes, representing the most common areas of a gene model. This means that most of the exons in the gene model will likely not have representation on the array, meaning that it is not practical to enable exon-level analysis.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the Clariom Assay scan times and file sizes?

Clariom D Assay - scan time is about 33 min.
- DAT file size about 790-800MB
- CEL file size about 68 MB

The Clariom S - scan time is about 5 min.
- DAT file size about 35-40MB
- CEL file size about 3 MB

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Is globin reduction of blood samples required when using the Clariom S assays?

No, globin reduction is not necessary for the Clariom S assay. The Clariom S assay is designed to preserve sample integrity and reduce data variability without requiring a globin or rRNA removal step. This means that you can perform the Clariom S assay without the need to perform globin reduction on your samples.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the correct fluidics script for the Clariom S (human, mouse and rat) array?

The Clariom S array is a 400 format, the correct script is FS450_0007.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How do I install Expression Console Software and TAC Software library files for human, mouse, or rat transcriptome assays?

Analysis library files and annotation files can be downloaded from within the TAC Software. Please refer to the respective user manuals for detailed instructions.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What software is available for analyzing the results from my human, mouse, or rat transcriptome assays?

Probe cell intensity data (CEL) from human, mouse, or rat transcriptome assays are analyzed in Transcriptome Analysis Console(TAC) Software. The application uses a version of RMA analysis to create CHP files. Transcriptome Analysis  Console Software can be downloaded at no charge from the website. Further statistical analysis may be performed in Affymetrix' Transcriptome Analysis Console (TAC) Software to obtain a list of differentially expressed genes and alternative splicing events. TAC Software also provides the visualization of genes, exons, junctions, transcript isoforms, pathways, and mRNA-miRNA regulatory networks.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

How do I install AGCC library files for the human, mouse, or rat transcriptome arrays?

To install library files for the human, mouse, or rat transcriptome arrays for AGCC, simply run the executable library file installer.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What format are the human, mouse, or rat transcriptome arrays?

Human, mouse, or rat transcriptome arrays are 49-format.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which fluidics protocol should I use when processing human, mouse, or rat transcriptome assays?

FS450_0001 should be used when processing human, mouse, or rat transcriptome assays. Up-to-date fluidics scripts can be obtained from the website.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can I analyze the results from my human, mouse, or rat transcriptome assays using GeneChip Operating Software (GCOS) or GeneChip Command Console Software (AGCC)?

Human, mouse, or rat transcriptome assays utilize an updated installer package that is not compatible with GCOS. Human, mouse, or rat transcriptome assays require AGCC for fluidics and scanning. The Expression Console Software is required to perform QC analysis of the human, mouse, or rat transcriptome array data. Expression Console Software can be downloaded at no charge from the website.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which protocol should I use to prepare my sample for hybridization to GeneChip human, mouse, or rat transcriptome assays?

Affymetrix human, mouse, and rat transcriptome assays are available in three formats based on sample type and amount of input RNA:

Human, mouse, and rat transcriptome Pico assays compatible with 100-2,000 pg of RNA isolated from fresh, frozen, blood, or FFPE samples
Human, mouse, and rat transcriptome FFPE assays compatible with 20-200 ng of RNA isolated from FFPE samples
Human, mouse, and rat transcriptome assays compatible with 50-500 ng of RNA isolated from fresh, frozen, or blood samples

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.