|Product Type||Antibody Biotinylation Kit|
|Labeling Scale||100 μg|
|Label or Dye||Biotin|
|Labeling Target||Antibodies (General)|
|Unit Size||8 reactions|
|Catalog Number||Specifications||Unit Size||Price (USD)||Availability||Quantity|
The Thermo Scientific™ Pierce™ Antibody Biotinylation Kit for IP provides biotinylation reagents designed specifically for the labeling of primary antibodies used in immunoprecipitation applications.
The kit contains sufficient reagents to label 50 to 200μg of antibody in 100μL reaction volumes for eight samples. The NHS-PEG4-biotin labeling reagent contains an amine-reactive N-hydroxysuccinimide ester (NHS) group and a water-soluble PEG4 spacer for optimal labeling and is provided in easy-to-use, single-use microtubes. Both the labeling efficiency of the biotinylation reagent and binding affinity of the labeled antibody have been validated using mouse monoclonal (IgG1, IgG2), rabbit polyconal, and rabbit monoclonal antibodies. Zeba™ Desalting Spin Columns are provided for easy and efficient removal of salts and excess biotin.