The Thermo Scientific™ Pierce™ MS-Compatible Magnetic IP Kit (Streptavidin) provides mass spectrometry-friendly reagents and an optimized protocol to enable highly effective and efficient immunoprecipitation and co-immunoprecipitation of target antigens upstream of LC-MS analysis.
• MS-compatible—reagents directly compatible with in-solution peptide digestion, enriched samples contain minimal detergent residuals detected using LC-MS • Sensitive—procedure successfully enriches low abundance proteins (low ng range) • Low background—binding, wash, and elution buffers optimized to minimize enrichment of background proteins • Reduced antibody contamination—antibody biotinylation reduces contamination in the eluate when compared to other IP methods • Robust—procedure and reagents have been robustly tested with numerous targets to ensure consistent enrichment of low abundant proteins (ng range) with at least 2 peptides identified per protein
The Pierce MS-Compatible Magnetic IP Kit (Streptavidin) uses high-quality Pierce Streptavidin Magnetic Beads and optimized buffers that are compatible with downstream LC-MS sample preparation and analysis. After the immunoprecipitation procedure, the target-enriched elution fraction is directly compatible with in-solution tryptic digestion, eliminating the need for gel purification, detergent removal, or desalting.
This kit has been rigorously validated using numerous target antigens with varying expression levels, including targets previously undetected without enrichment or by western blotting. To support IP and co-IP applications using streptavidin supports, the Pierce Antibody Biotinylation Kit for IP has been specifically designed to enable biotin labeling of antibodies. Additionally, the reagents and procedures have been validated using both manual and automated magnetic separation.
Applications: • Enrichment of low abundance targets to improve MS sensitivity • Identification of target proteins that are not detectable by western blotting • Quantitation of target proteins by LC-MS analysis • Enrichment of target proteins from plasma samples for MS analysis
For Research Use Only. Not for use in diagnostic procedures.