Applied Biosystems™

Axiom™ PharmacoFocus™ Assay Core Kit, mini 96-array

Catalog number:  952425

Related applications:

Microarray Analysis

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The Axiom PharmacoFocus Assay Core Kit offers comprehensive coverage of targeted high-evidence pharmacogenomic research content (Pharmacogenomics Knowledge Base annotation levels of evidence 1A-2B) with a cost-efficient and high-throughput solution. The panel includes 2,000 variants in 150 genes and variants in regions of high homology to pseudo-genes (such as cytochrome P450 (CYP) genes), covers population diversity, and offers high accuracy and reproducibility.

The assay offers genotyping, copy number, and HLA typing, achieving the task of three different technologies in a single workflow at a cost per sample that enables profiling of hundreds to hundreds of thousands of samples. The assay is ideal for pharmacogenomic research in labs, academic hospitals, and health care centers, and for pharmaceutical companies with research applications in clinical trials.

Each Axiom PharmacoFocus Assay Core Kit, mini 96-array, includes all necessary array plates, reagents, controls, and consumables to process 94 samples and 2 controls. For a kit that offers star allele reporting with predicted gene phenotype, please see the Axiom PharmacoFocus Assay Kit, mini 96-array.

Features of the Axiom PharmacoFocus Assay Core Kit include:
• 2,000 markers in 150 genes of known pharmacogenomic value
• 88% of markers with PharmGKB annotation levels 1A-2B
• Accurate genotyping of markers in regions of high homology within CYP2C19, CYP2C8, CYP2D6, CYP2A6, CYP2B6, GSTM1, and SULT1A1
• Copy number variation (CNV) analysis for key ADME genes: CYP2D6, CYP2A6, GSTM1, GSTT1, UGB2B17, and SULT1A1
• HLA analysis with high resolution typing (2- and 4-digit) of the major histocompatibility complex (3 MHC Class I and 8 Class II loci) including HLA-A* and HLA-B* types with relevance in drug metabolism

The Axiom PharmacoFocus Assay Core Kit includes:
• Pharmacogenomic markers with highest PharmGKB levels of evidence (levels 1A, 1B, 2A, 2B) accessed in Nov 2019. Majority of annotations are associated with toxicity/adverse drug reactions and efficacy.
• Dutch Pharmacogenetics Working Group passport markers (accessed in Nov 2019)
• OpenArray TaqMan panels markers
• Markers referenced in the Center for Medicare and Medicaid (CMS) reimbursement policy including Psychiatry panel
• Human leukocyte antigen (HLA) markers associated with drug reactions

The Axiom PharmacoFocus Assay Core Kit requires the Applied Biosystems GeneTitan Multi-Channel Instrument, a platform used worldwide by genetic researchers and known for an efficient workflow and high reproducibility. Axiom arrays are manufactured using photolithographic manufacturing technology known for 100% fidelity, ensuring all markers are available in any manufacturing batch.

The Axiom PharmacoFocus Assay Core Kit data is analyzed with Applied Biosystems Axiom Analysis Suite software, which offers genotyping and copy number analysis. The Axiom HLA Analysis software can also be used for HLA typing.


Array: Genotyping Microarrays
Array Format: mini 96-array format
Number of Arrays: 96
Species: Human
Type: Assay Core Kit

Contents & storage

Sufficient for processing one mini 96-array plate
• 1 Axiom PharmacoFocus Array Core plate, mini 96-array format; Store at 2-8°
• 1 reagent kit (includes reagents and controls for 1 x mini 96-array format plate). Storage can vary for each reagent; refer to product insert.
• Gene Titan instrument consumables for 1 x mini 96-array format plate. Store at room temperature.