StemPro® hESC SFM

Catalog number: A1000701

Gibco™  Related applications: Clinical & Translational Research | Mammalian Cell Culture | Stem Cell Culture

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Description

Developed for human embryonic and human induced pluripotent stem cell culture

StemPro® hESC SFM is a fully defined, serum- and feeder-free medium (SFM) specially formulated for the growth and expansion of human embryonic (hESCs) and human induced pluripotent stem cells (iPSC). StemPro® hESC SFM has been extensively tested and is proven to maintain pluripotency in a growing list of hESC lines including BG01, BG02, BG03, H1, H9, and BG01V. In addition, StemPro® hESC SFM has been used to scale up production of hESCs to over 1 x 1010 cells. StemPro® hESC SFM has been shown to support hESC and iPSC growth for >50 passages without any signs of karyotypic abnormalities, and to maintain the ability of hESCs to differentiate into all three germ line lineages.

StemPro® hESC SFM Components:
StemPro® hESC Supplement
DMEM⁄F-12 with GlutaMAX™ medium
Bovine serum albumin 25% (BSA)
Additional components required for complete medium (need to be purchased separately):
FGF BASIC (full length) REC HU (Cat. no. PHG0261)
2-Mercaptoethanol (Cat. no. 21985-023)

Applications:
Formulated for the growth and expansion of human embryonic and human induced pluripotent stem cells.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Stem Cells (Embryonic)
Form: Liquid
Species: Human
Serum Level: Serum-Free
Culture Type: Feeder Cell-Free
Product Size: 1 kit
Classification: Serum-Free

Contents & storage

StemPro® hESC Supplement: Store at -5 to -20°C (in the dark). DMEM/F-12 with GlutaMAX™ medium: Store at 2 to 8°C (protect from light). Bovine serum albumin 25% (BSA): Store at 2 to 8°C (protect from light). These components are guaranteed stable for 12 months when properly stored.

Note: The complete StemPro® hESC SFM (supplement, DMEM/F-12, BSA, FGF basic, and 2-mercaptoethanol) is stable for 1 day when stored in the dark at 2-8°C. We recommend preparing a minimal volume of the complete working medium (enough for the day) and pre-equilibrating with respect to temperature and gases just prior to use.

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