Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)

Citations & References (10)

Invitrogen™

Red/Green BODIPY™ PC-A2 (1-O-(6-BODIPY™ 558/568-Aminohexyl)-2-BODIPY™ FL C5-Sn-Glycero-3-Phosphocholine) (Ratiometric Phospholipase A2 Substrate)

Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cellRead more

Catalog Number	Quantity
A10072	100 μg

Catalog number A10072

Price (MXN)

Quantity:

100 μg

Molecular Probes™ phospholipase substrates are dye labeled phospholipids used to monitor phospholipase A (PLA) activity in purified enzyme preparations, cell lysates, and living cells. Two structural variations of these substrates are available, which confer specificity for PLA1 and PLA2. Fluorescently labeled versions of the cleavage product generated from these substrates are also available for use as standards for evaluating percentage substrate conversion in enzymatic reactions.

Fluorogenic Phospholipase A Substrates Specifications:

• Label (Ex/Em): Red/Green BODIPY™ PC-A2 (∼488/530,590 nm) (can be used in dual emission fluorescence ratio detection)
• Specificity: PLA₂
• Cleavage product: D3834
• Lyophilized product can be dissolved in DMSO for use
• Fluorescence is measured either in microplate assays or using flow cytometry.

Find More Probes for Lipid Metabolism and Fatty Acid Analogs
Review Fatty Acid Analogs and Phospholipids—Section 13.2 and Probes for Lipid Metabolism and Signaling—Section 17.4 in the Molecular Probes™ Handbook for more information on these products.

For Research Use. Not for human or animal therapeutic or diagnostic use.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell PermeabilityCell-Permeant

For Use With (Equipment)Flow Cytometer, Microplate Reader

Product LineBODIPY

Quantity100 μg

Shipping ConditionRoom Temperature

SubstratePhospholipase A2 substrate (ratiometric)

Detection MethodFluorescence

FormSolid

Substrate PropertiesLipid-Based Substrate

Target EnzymePhospholipase

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C).

Frequently asked questions (FAQs)

The FRET-based substrate Red/Green BODIPY PC-A2 shows no change in fluorescence upon enzymatic cleavage. I'm not seeing an increasing green signal with higher concentrations of phospholipase A2 and no change in red fluorescence. Why is this?

There are numerous factors that can interfere with FRET-based detection. Here are a few items to consider:

a) Is there any component in the reaction solution that can quench green fluorescence?
b) Is there any component in the reaction solution that autofluoresces in the green or red emission range?
c) Too high of a substrate concentration may result in diffusional FRET: Lower the final working concentration of the substrate.
d) Ensure adequate dispersal of the substrate during the reaction. BODIPY dyes are very hydrophobic, and aggregates of the green product, BODIPY FL, can form an excimer and emit in the red range.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

You provide Phospholipase A2 from honey bee venom in the EnzChek Phospholipase A2 Assay Kit. Can the kit be used with phospholipase A2 from other species?

Yes, phospholipase A2 from other species may be used as long as the phospholipase A2 can hydrolyze the sn-2 ester linkage of phospholipids and the enzymatic reaction can be done within a pH of >3 and <9.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (10)

Citations & References

Abstract

Inhibition of Cytosolic Phospholipase A2α Impairs an Early Step of Coronavirus Replication in Cell Culture.

Authors:

Journal:J Virol

PubMed ID:29167338

Tentonin 3/TMEM150C regulates glucose-stimulated insulin secretion in pancreatic β-cells.

Authors:

Journal:Cell Rep

PubMed ID:34852221

Injectable nanohydroxyapatite-chitosan-gelatin micro-scaffolds induce regeneration of knee subchondral bone lesions.

Authors:

Journal:Sci Rep

PubMed ID:29196647

Structure/function relationships of adipose phospholipase A2 containing a cys-his-his catalytic triad.

Authors:

Journal:J Biol Chem

PubMed ID:22923616

The lipid transporter Mfsd2a maintains pulmonary surfactant homeostasis.

Authors:

Journal:J Biol Chem

PubMed ID:35150739

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