Thermo Scientific™

qualyfast™ Legionella PCR and DNA Extraction Kits

Catalog number: A101013
Thermo Scientific™

qualyfast™ Legionella PCR and DNA Extraction Kits

Catalog number: A101013
Detect both Legionella species and Legionella pneumophila from water samples with this rapid, sensitive and specific solution enabled by the qualyfast® Legionella PCR Assay, Free DNA Inactivator, DNA Extraction and Quantification Standards kits.The assay independently detects both Legionella species and L. pneumophilia, includes inhibition control to minimize false-negative results, and offers the option for quantification by using calibrated standards. All consumables required for the workflow are included within the kits, with lyophilized and pre-dosed PCR reagents for ease-of-use.
 
Catalog Number
A101013
Unit Size
Each
Description
qualyfast Legionella PCR Assay
Price (USD)
Full specifications
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests24 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast Legionella PCR Assay
Price (USD)
Full specifications
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests48 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast Legionella PCR Assay
Price (USD)
Full specifications
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests96 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast Legionella Quantification Standards 8 tests
Price (USD)
Full specifications
Descriptionqualyfast Legionella Quantification Standards 8 tests
For Use With (Application)PCR/DNA Extraction
No. of Tests1 Kit x 8 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast DNA Extraction Kit I – Clear Water
Price (USD)
Full specifications
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 24 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast DNA Extraction Kit I – Clear Water
Price (USD)
Full specifications
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 48 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast DNA Extraction Kit II – Dirty Water
Price (USD)
Full specifications
Descriptionqualyfast DNA Extraction Kit II – Dirty Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 48 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast DNA Extraction Kit I – Clear Water
Price (USD)
Full specifications
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 96 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast DNA Extraction Kit II – Dirty Water
Price (USD)
Full specifications
Descriptionqualyfast DNA Extraction Kit II – Dirty Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 96 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Catalog Number
Unit Size
Each
Description
qualyfast free DNA removal
Price (USD)
Full specifications
Descriptionqualyfast free DNA removal
For Use With (Application)PCR/DNA Extraction
No. of Tests200 Reactions
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For200 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 200 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Showing 10 of 10
Catalog NumberSpecificationsUnit SizeDescriptionPrice (USD)
A101013Full specifications
Eachqualyfast Legionella PCR AssayRequest A Quote
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests24 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
A201013Full specifications
Eachqualyfast Legionella PCR AssayRequest A Quote
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests48 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
A301013Full specifications
Eachqualyfast Legionella PCR AssayRequest A Quote
Descriptionqualyfast Legionella PCR Assay
For Use With (Application)PCR/DNA Extraction
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests96 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodPrimer-Probe
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
A501013Full specifications
Eachqualyfast Legionella Quantification Standards 8 testsRequest A Quote
Descriptionqualyfast Legionella Quantification Standards 8 tests
For Use With (Application)PCR/DNA Extraction
No. of Tests1 Kit x 8 tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions8 Reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D101001Full specifications
Eachqualyfast DNA Extraction Kit I – Clear WaterRequest A Quote
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 24 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D201001Full specifications
Eachqualyfast DNA Extraction Kit I – Clear WaterRequest A Quote
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 48 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D211001Full specifications
Eachqualyfast DNA Extraction Kit II – Dirty WaterRequest A Quote
Descriptionqualyfast DNA Extraction Kit II – Dirty Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 48 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D301001Full specifications
Eachqualyfast DNA Extraction Kit I – Clear WaterRequest A Quote
Descriptionqualyfast DNA Extraction Kit I – Clear Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 96 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D311001Full specifications
Eachqualyfast DNA Extraction Kit II – Dirty WaterRequest A Quote
Descriptionqualyfast DNA Extraction Kit II – Dirty Water
For Use With (Application)PCR/DNA Extraction
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 96 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
D401009Full specifications
Eachqualyfast free DNA removalRequest A Quote
Descriptionqualyfast free DNA removal
For Use With (Application)PCR/DNA Extraction
No. of Tests200 Reactions
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For200 Reactions
Detection MethodFluorescence
FormLyophilized Powder
FormatTube
GC-Rich PCR PerformanceLow
No. of Reactions1 Kit x 200 reactions
PCR MethodqPCR
Reaction SpeedFast
Unit SizeEach
Showing 10 of 10

Minimizes false negatives

  • Free DNA Inactivator removes DNA from non-viable sources commonly found in environmental water samples.
  • DNA Extraction Kits remove environmental contaminants minimizing potential inhibition effects.
  • PCR Assays with simultaneous co-amplification of an Internal Application Control allowing determination of potential inhibition effects.

Increases accuracy

  • Quantification Standards enable PCR detection of Legionella species to be quantified within the 25-25,000 Genomic Unit range.
  • Increases throughput: Multiplexed PCR of the characteristic DNA sequences for species within the Legionella genus and the specific species L. pneumophilia.

Easy-to-use

  • PCR Assay kits contain strips with eight reaction tubes containing lyophilized PCR reagents, DNA free solution and pre-dosed positive-control tubes.
  • Quantification Standards are pre-aliquoted and lyophilized in individual tubes.
  • DNA Extraction Kits contain all the consumables required to extract DNA from environmental water samples.

Optimized workflow

  • Complete end-to-end workflow covering sample preparation using DNA Extraction Kits and Free DNA Inactivator, detection using multiplex Legionella PCR Assay and evaluation of results using Quantification Standards
  • ISO compliant: workflow is compliant with the protocol, experimental design and calculation methods described by ISO/TS 12869:2012

The qualyfast® Legionella PCR Assay contains:

  •  Pre-dosed and lyophilized PCR reagents in strips of 8 reaction tubes
  • A strip of 8 reaction tubes containing positive controls
  • DNA-free solution


The qualyfast® DNA Extraction Kit I
is designed for use with clear water samples, as acquired from water distribution systems, drinking water and other sources that contain minimal suspended particles. The kit contains:

  • Single-use, sterile membrane filters
  • Lysis buffer for enhanced isolation of microorganisms from filter membranes
  • Lysis enzyme for DNA extraction


The qualyfast® DNA Extraction Kit II
is designed for use with dirty water, biofilm and sediment samples. The kit contains:

  • Single-use, sterile membrane filters
  • Pre-lysis buffer
  • Lysis solution & bead beating lysis tubes
  • Spin filter
  • Post-lysis filtration columns and reagents
  • All collection tubes required for sample handling during extraction

The qualyfast® Free DNA Inactivator Kit contains:

  • DNA inactivation enzyme (lyophilized)
  • DNA inactivation buffers

The qualyfast® Legionella Quantification Standards Kit contains:

  • Four different quantified standards, each packaged in strips of 8 reaction tubes, enabling 8 analysis sessions
  • DNA-free solution for reconstitution of lyophilized reagents and negative-control

The qualyfast® Legionella workflow is compatible with the Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System or Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System.

qualyfast is a trademark ofBioside S.r.l, Via Albert Einstein, snc Località Cascina Codazza c/o Parco Tecnologico Padano26900 Lodi (LO) ITALY
For Laboratory Use

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Frequently asked questions (FAQs)

Citations & References