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qualyfast™ Legionella PCR and DNA Extraction Kits
Rapidly detect Legionella species and Legionella pneumophila from water samples using real-time qPCR
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| Catalog Number | Description | No. of Reactions | No. of Tests |
|---|---|---|---|
| A101013 | Qualyfast Legionella PCR Assay | 8 Reactions | 24 Tests |
| A201013 | Qualyfast Legionella PCR Assay | 8 Reactions | 48 Tests |
| A301013 | Qualyfast Legionella PCR Assay | 8 Reactions | 96 Tests |
| A501013 | Qualyfast Legionella Quantification Standards 8 tests | 8 Reactions | 8 Tests |
| D101001 | Qualyfast DNA Extraction Kit I – Clear Water | 24 Reactions | |
| D201001 | Qualyfast DNA Extraction Kit I – Clear Water | 48 Reactions | |
| D211001 | Qualyfast DNA Extraction Kit II – Dirty Water | 48 Reactions | |
| D301001 | Qualyfast DNA Extraction Kit I – Clear Water | 96 Reactions | |
| D311001 | Qualyfast DNA Extraction Kit II – Dirty Water | 96 Reactions | |
| D401009 | Qualyfast free DNA removal | 200 Reactions |
Catalog number A101013
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Description:
Qualyfast Legionella PCR Assay
No. of Reactions:
8 Reactions
No. of Tests:
24 Tests
Detect both Legionella species and Legionella pneumophila from water samples with this rapid, sensitive and specific solution enabled by the qualyfast™ Legionella PCR Assay, Free DNA Inactivator, DNA Extraction and Quantification Standards kits.The assay independently detects both Legionella species and L. pneumophilia, includes inhibition control to minimize false-negative results, and offers the option for quantification by using calibrated standards. All consumables required for the workflow are included within the kits, with lyophilized and pre-dosed PCR reagents for ease-of-use.
Minimizes false negatives
- Free DNA Inactivator removes DNA from non-viable sources commonly found in environmental water samples.
- DNA Extraction Kits remove environmental contaminants minimizing potential inhibition effects.
- PCR Assays with simultaneous co-amplification of an Internal Application Control allowing determination of potential inhibition effects.
Increases accuracy
- Quantification Standards enable PCR detection of Legionella species to be quantified within the 25-25,000 Genomic Unit range.
- Increases throughput: Multiplexed PCR of the characteristic DNA sequences for species within the Legionella genus and the specific species L. pneumophilia.
Easy-to-use
- PCR Assay kits contain strips with eight reaction tubes containing lyophilized PCR reagents, DNA free solution and pre-dosed positive-control tubes.
- Quantification Standards are pre-aliquoted and lyophilized in individual tubes.
- DNA Extraction Kits contain all the consumables required to extract DNA from environmental water samples.
Optimized workflow
- Complete end-to-end workflow covering sample preparation using DNA Extraction Kits and Free DNA Inactivator, detection using multiplex Legionella PCR Assay and evaluation of results using Quantification Standards
- ISO compliant: workflow is compliant with the protocol, experimental design and calculation methods described by ISO/TS 12869:2012
The qualyfast™ Legionella PCR Assay contains:
- Pre-dosed and lyophilized PCR reagents in strips of 8 reaction tubes
- A strip of 8 reaction tubes containing positive controls
- DNA-free solution
The qualyfast™ DNA Extraction Kit I is designed for use with clear water samples, as acquired from water distribution systems, drinking water and other sources that contain minimal suspended particles. The kit contains:
- Single-use, sterile membrane filters
- Lysis buffer for enhanced isolation of microorganisms from filter membranes
- Lysis enzyme for DNA extraction
The qualyfast™ DNA Extraction Kit II is designed for use with dirty water, biofilm and sediment samples. The kit contains:
- Single-use, sterile membrane filters
- Pre-lysis buffer
- Lysis solution & bead beating lysis tubes
- Spin filter
- Post-lysis filtration columns and reagents
- All collection tubes required for sample handling during extraction
The qualyfast™ Free DNA Inactivator Kit contains:
- DNA inactivation enzyme (lyophilized)
- DNA inactivation buffers
The qualyfast™ Legionella Quantification Standards Kit contains:
- Four different quantified standards, each packaged in strips of 8 reaction tubes, enabling 8 analysis sessions
- DNA-free solution for reconstitution of lyophilized reagents and negative-control
The qualyfast™ Legionella workflow is compatible with the Applied Biosystems™ 7500 Fast Food Safety Real-Time PCR System or Applied Biosystems™ QuantStudio™ 5 Food Safety Real-Time PCR System.
qualyfast is a trademark ofBioside S.r.l, Via Albert Einstein, snc Località Cascina Codazza c/o Parco Tecnologico Padano26900 Lodi (LO) ITALY
For Laboratory Use
Specifications
DescriptionQualyfast Legionella PCR Assay
Detection MethodPrimer-probe
FormLyophilized
FormatTube
GC-Rich PCR PerformanceLow
PCR MethodqPCR
Reaction SpeedFast
For Use With (Equipment)Applied Biosystems 7500 Fast Food Safety Real-Time PCR instrument and Applied Biosystems QuantStudio 5 Food Safety Real Time PCR System
No. of Tests24 Tests
Product TypeLegionella PCR and DNA Extraction Kit
Sufficient For8 Reactions
For Use With (Application)Pathogen Detection, DNA Extraction
No. of Reactions8 Reactions
Unit SizeEach