Alexa Fluor™ 546 Protein Labeling Kit, 1 kit - Citations
Alexa Fluor™ 546 Protein Labeling Kit, 1 kit - Citations
View additional product information for Antibody Labeling Kits for 1 mg - Citations (A10235, A10236, A10237, A10238, A20170, A10239, A20173, P30012, A20174, A20171, A20172, D20655, F10240, O10241, A10170)
Showing 18 product Citations
Citations & References
Abstract
Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates.
AuthorsPanchuk-Voloshina N, Haugland RP, Bishop-Stewart J, Bhalgat MK, Millard PJ, Mao F, Leung WY, Haugland RP
JournalJ Histochem Cytochem
PubMed ID10449539
'Alexa 350, Alexa 430, Alexa 488, Alexa 532, Alexa 546, Alexa 568, and Alexa 594 dyes are a new series of fluorescent dyes with emission/excitation spectra similar to those of AMCA, Lucifer Yellow, fluorescein, rhodamine 6G, tetramethylrhodamine or Cy3, lissamine rhodamine B, and Texas Red, respectively (the numbers in the ... More
Antibody interactions with the capsule of Cryptococcus neoformans.
AuthorsFeldmesser M, Rivera J, Kress Y, Kozel TR, Casadevall A
JournalInfect Immun
PubMed ID10816523
'Monoclonal antibodies to the encapsulated fungus Cryptococcus neoformans produce different immunofluorescence (IF) patterns after binding to the polysaccharide capsule. To explore the relationship between the IF pattern and the location of antibody binding, two immunoglobulin M (IgM) monoclonal antibodies (MAbs) (12A1 and 13F1) that differ in protective efficacy and IF ... More
Temporally resolved interactions between antigen-stimulated IgE receptors and Lyn kinase on living cells.
AuthorsLarson DR, Gosse JA, Holowka DA, Baird BA, Webb WW
JournalJ Cell Biol
PubMed ID16275755
'Upon cross-linking by antigen, the high affinity receptor for immunoglobulin E (IgE), FcepsilonRI, is phosphorylated by the Src family tyrosine kinase Lyn to initiate mast cell signaling, leading to degranulation. Using fluorescence correlation spectroscopy (FCS), we observe stimulation-dependent associations between fluorescently labeled IgE-FcepsilonRI and Lyn-EGFP on individual cells. We also ... More
Serodiagnosis of infectious diseases with antigen microarrays.
AuthorsBacarese-Hamilton T, Mezzasoma L, Ardizzoni A, Bistoni F, Crisanti A
JournalJ Appl Microbiol
PubMed ID14678154
'AIMS: To generate protein microarrays by printing microbial antigens on slides to enable the simultaneous determination in human sera of antibodies directed against Toxoplasma gondii, rubella virus, cytomegalovirus and herpes simplex virus (HSV) types 1 and 2. METHODS AND RESULTS: Antigens were printed on activated glass slides using high-speed robotics. ... More
EliCell: a gel-phase dual antibody capture and detection assay to measure cytokine release from eosinophils.
AuthorsBandeira-Melo C, Gillard G, Ghiran I, Weller PF
JournalJ Immunol Methods
PubMed ID11033023
'Eosinophils contain many preformed cytokines and chemokines, which are stored in specific granules along with cationic granule proteins. Mobilization and release of these granule contents can be selective and mediated by vesicular transport. We have developed a sensitive method to detect and quantitate eosinophil vesicular transport-mediated release of specific eosinophil ... More
Fluorescent histochemical techniques for analysis of intracellular signaling.
AuthorsOksvold MP, Skarpen E, Widerberg J, Huitfeldt HS
JournalJ Histochem Cytochem
PubMed ID11850432
Intracellular signaling relies on the orchestrated cooperation of signaling proteins and modules, their intracellular localization, and membrane trafficking. Recently, a repertoire of fluorescence-based techniques, which significantly increases our potential for detailed studies of the involved mechanisms, has been introduced. Microscopic techniques with increased resolution have been combined with improved techniques ... More
Molecular characterization of proprotein convertase subtilisin/kexin type 9-mediated degradation of the LDLR.
AuthorsWang Y, Huang Y, Hobbs HH, Cohen JC,
JournalJ Lipid Res
PubMed ID22764087
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secreted protein that promotes degradation of cell surface LDL receptors (LDLRs) in selected cell types. Here we used genetic and pharmacological inhibitors to define the pathways involved in PCSK9-mediated LDLR degradation. Inactivating mutations in autosomal recessive hypercholesterolemia (ARH), an endocytic adaptor, blocked ... More
The tumor necrosis factor receptor stalk regions define responsiveness to soluble versus membrane-bound ligand.
AuthorsRichter C, Messerschmidt S, Holeiter G, Tepperink J, Osswald S, Zappe A, Branschädel M, Boschert V, Mann DA, Scheurich P, Krippner-Heidenreich A,
JournalMol Cell Biol
PubMed ID22547679
The family of tumor necrosis factor receptors (TNFRs) and their ligands form a regulatory signaling network that controls immune responses. Various members of this receptor family respond differently to the soluble and membrane-bound forms of their respective ligands. However, the determining factors and underlying molecular mechanisms of this diversity are ... More
Binding of laminin-1 to monosialoganglioside GM1 in lipid rafts is crucial for neurite outgrowth.
AuthorsIchikawa N, Iwabuchi K, Kurihara H, Ishii K, Kobayashi T, Sasaki T, Hattori N, Mizuno Y, Hozumi K, Yamada Y, Arikawa-Hirasawa E,
JournalJ Cell Sci
PubMed ID19118221
Laminin-1, an extracellular matrix molecule, promotes neurite outgrowth through the interaction of integrin and actin. Monosialoganglioside GM1 in the lipid rafts associates with and activates the NGF receptor TrkA, and enhances neurite outgrowth. However, the role of GM1 in laminin-1-induced neurite outgrowth was still unclear. Here, we describe that laminin-1 ... More
Real-time imaging of fluorescent flagellar filaments.
AuthorsTurner L, Ryu WS, Berg HC
JournalJ Bacteriol
PubMed ID10781548
Bacteria swim by rotating flagellar filaments that are several micrometers long, but only about 20 nm in diameter. The filaments can exist in different polymorphic forms, having distinct values of curvature and twist. Rotation rates are on the order of 100 Hz. In the past, the motion of individual filaments ... More
RET finger protein is a transcriptional repressor and interacts with enhancer of polycomb that has dual transcriptional functions.
AuthorsShimono Y, Murakami H, Hasegawa Y, Takahashi M
JournalJ Biol Chem
PubMed ID10976108
RET finger protein (RFP) belongs to the large B-box RING finger protein family and is known to become oncogenic by fusion with RET tyrosine kinase. Although RFP is reported to be a nuclear protein that is present in the nuclear matrix, its function is largely unknown. Here we show that ... More
Predominant role of toll-like receptor 2 versus 4 in Chlamydia pneumoniae-induced activation of dendritic cells.
AuthorsPrebeck S, Kirschning C, Dürr S, da Costa C, Donath B, Brand K, Redecke V, Wagner H, Miethke T
JournalJ Immunol
PubMed ID11544320
Chlamydia pneumoniae is an obligate intracellular human pathogen causing diseases such as pneumonia, bronchitis, and pharyngitis. Because of its intracellular replication, cell-mediated immune responses are needed to mediate successful defenses of the host. Because dendritic cells play a central role in linking innate immunity and Ag-specific cell-mediated immune responses we ... More
Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition.
Seven-color analyses of immunofluorescence-stained tissue samples were accomplished using Fourier spectroscopy-based hyperspectral imaging and singular value decomposition. This system consists of a combination of seven fluorescent dyes, three filtersets, an epifluorescence microscope, a spectral imaging system, a computer for data acquisition, and data analysis software. The spectra of all pixels ... More
In vivo imaging of germinal centres reveals a dynamic open structure.
AuthorsSchwickert TA, Lindquist RL, Shakhar G, Livshits G, Skokos D, Kosco-Vilbois MH, Dustin ML, Nussenzweig MC
JournalNature
PubMed ID17268470
Germinal centres are specialized structures wherein B lymphocytes undergo clonal expansion, class switch recombination, antibody gene diversification and affinity maturation. Three to four antigen-specific B cells colonize a follicle to establish a germinal centre and become rapidly dividing germinal-centre centroblasts that give rise to dark zones. Centroblasts produce non-proliferating centrocytes ... More
Use of quantum dot luminescent probes to achieve single-cell resolution of human oral bacteria in biofilms.
AuthorsChalmers NI, Palmer RJ, Du-Thumm L, Sullivan R, Shi W, Kolenbrander PE
JournalAppl Environ Microbiol
PubMed ID17114321
Oral biofilms are multispecies communities, and in their nascent stages of development, numerous bacterial species engage in interspecies interactions. Better insight into the spatial relationship between different species and how species diversity increases over time can guide our understanding of the role of interspecies interactions in the development of the ... More
The role of chemokines in the microenvironmental control of T versus B cell arrest in Peyer's patch high endothelial venules.
AuthorsWarnock RA, Campbell JJ, Dorf ME, Matsuzawa A, McEvoy LM, Butcher EC
JournalJ Exp Med
PubMed ID10620606
Chemokines have been hypothesized to contribute to the selectivity of lymphocyte trafficking not only as chemoattractants, but also by triggering integrin-dependent sticking (arrest) of circulating lymphocytes at venular sites of extravasation. We show that T cells roll on most Peyer's patch high endothelial venules (PP-HEVs), but preferentially arrest in segments ... More
CD11c+ eosinophils in the murine thymus: developmental regulation and recruitment upon MHC class I-restricted thymocyte deletion.
AuthorsThrosby M, Herbelin A, Pléau JM, Dardenne M
JournalJ Immunol
PubMed ID10925279
Eosinophils are bone marrow-derived cells released into the circulation during hypersensitivity reactions and parasitic infections. Under normal conditions most eosinophils are tissue bound, where their physiologic role is unclear. During in situ analysis of the thymic microenvironment for CD11c+ dendritic cell subpopulations (APC critical in the process of thymic negative ... More
Charge- and size-based separation of macromolecules using ultrathin silicon membranes.
Commercial ultrafiltration and dialysis membranes have broad pore size distributions and are over 1,000 times thicker than the molecules they are designed to separate, leading to poor size cut-off properties, filtrate loss within the membranes, and low transport rates. Nanofabricated membranes have great potential in molecular separation applications by offering ... More