CHO CD EfficientFeed™ B Liquid Nutrient Supplement This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  A1024001

Related applications:


| Cell Culture

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Gibco® CHO CD EfficientFeed™ B liquid nutrient supplement is a product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B liquid nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.

The liquid format is designed for small scale use and experimentation. This product is also available in AGT™ format for large scale manufacturing and ease of use.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Ready for larger scale use with convenient packaging options (10 L and 100 L)

Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0

Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium

Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium

Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0

Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including less consistent hydrolysates (Figures 2, 3a & 3b).

Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ B AGT™ supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L liquid supplement as a seamless process, as well as allow for cost savings when purchasing production level volumes.

Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ B supplement (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.

AGT™ Format Ease of Use
CHO CD EfficientFeed™ B AGT™ Nutrient Supplement delivers dry format scale-up convenience as well as simple reconstitution in a single part AGT dry format. For more details, consult the supporting documents provided on the AGT™ format product page.

For research or further manufacturing use. Not for use in diagnostic procedures.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


Cell Line: CHO
Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Culture Type: Suspension Cell Culture
Endotoxin Level: Low
Format: Bottle(s)
Green Features: Sustainable packaging
Included Antibiotics: No antibiotics
Product Line: Gibco®
Purity or Quality Grade: Cell Culture Grade, Qualified for Further Manufacturing, Research Grade
Remarks: Chemically Defined Nutrient Feeding Supplement
Serum Level: Serum-Free
Shipping Condition: Room Temperature
Sodium Bicarbonate Buffer: Sodium Bicarbonate
Sodium Pyruvate Additive: Sodium Pyruvate
Tested For: pH
Volume (Metric): 1 L
Form: Liquid
Glutamine: No Glutamine
Phenol Red Indicator: No Phenol Red
With Additives: No Insulin
Sterility: Sterile

Contents & storage

Contains one liter of CHO CD EfficientFeed™ B and a manual.
Store CHO CD EfficientFeed™ B at 2–8°C in the dark.