One Shot® ccdB Survival™ 2 T1^R Competent Cells

Description

One Shot® ccdB Survival™ 2 T1^R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 10⁹ transformants/µg plasmid DNA. The ccdB Survival™ 2 T1^R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1^R E. coli strain offers the following features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Str^R) endA1 nupG fhuA::IS2

Specifications

Competent Cell Type:	Chemically Competent
Species:	E. coli
Format:	One Shot
Transformation Efficiency:	> 1x10^9
Bacterial or Yeast Strain:	ccdB Survival™ 2
Fast Growth:	No
Product Size:	10 transformations
Blue-White Screening:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Library Construction:	No
Reduces Recombination:	Yes
Cloning Methylated DNA:	Yes
Improves Plasmid Quality:	Yes
Propagating ccdB Vectors:	Suitable for ccdB vector propagation
F' Episome (to make ssDNA):	Lacks F' Episome
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
T1 Phage - Resistant (tonA):	Yes
High Throughput Compatibility:	Not High Throughput-Compatible (Manual)
Shipping Condition:	Dry Ice

Contents & storage