Invitrogen™

One Shot™ ccdB Survival™ 2 T1R Competent Cells

Catalog number: A10460
Invitrogen™

One Shot™ ccdB Survival™ 2 T1R Competent Cells

Catalog number: A10460
Catalog Number
A10460
Unit Size
11 x 50 µL/tube
Price (USD)
Price: 337.00
Online price: 310.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1046011 x 50 µL/tube
Price: 337.00
Online price: 310.65
Your price:
-
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One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors. ccdB Survival 2 T1R cells that are made chemically competent can achieve high transformation efficiency of >1 x 109 transformants/μg plasmid DNA.

The ccdB Survival 2 T1R E. coli strain is derived from the TOP10 strain and offers the same benefits. Mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) allow cloning of both prokaryotic and eukaryotic genomic DNA that contains methylcytosine and methyladenine. This strain also has the lacZΔM15 genotype, providing the option of blue-white screening on plates containing either X-Gal or Bluo-Gal. In addition, recA1 and endA1 mutations increase insert stability and improve the quality of plasmid DNA prepared from minipreps.The tonA (also known as fhuA) mutation in ccdB Survival 2 T1R cells confers resistance to bacteriophages T1 and T5, safeguarding samples against contamination.

ccdB Survival 2 T1R Competent Cells offer:
• >1 x 109 transformants/μg efficiency
tonA (fhuA) genotype to confer resistance to T1 and T5 phage
• Support of high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype) 

Convenient and efficient format
One Shot ccdB Survival 2 T1R E. coli are supplied in the convenient, single-reaction One Shot format. Each tube contains enough cells for one transformation so there are no efficiency-zapping, freeze-thaw cycles or money wasted on unused cells.

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL(StrR) endA1 nupG fhuA::IS2

Find the strain and format that fits your needs
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
MultiShot formats for high throughput applications are available.
Explore bacterial growth media formats.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Chemically Competent Cells
Contains F' Episome
No
Improves Plasmid Quality
Yes (endA1)
Cloning Methylated DNA
Yes (mcrA)
Transformation Efficiency Level
High Efficiency (>1 x 109 cfu/μg)
Antibiotic Resistance Bacterial
Yes (Streptomycin)
Cloning Unstable DNA
Not suitable for cloning unstable DNA
Blue/White Screening
Yes (lacZΔM15)
High-throughput Compatibility
Low
Plasmid
ccdB vector propagation
Preparing Unmethylated DNA
No
Reduces Recombination
Yes (recA1)
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
Yes
Species
E. coli (K12)
Format
Tube
Product Line
One Shot™
Quantity
11 x 50 μL

Contents & Storage

• One Shot ccdB Survival 2 T1R Competent Cells (11 x 50 μL)
Store Competent Cells at &ndash80°C.

• pUC19 DNA (50 μL at 10 pg/μL)
Store pUC19 DNA at –20°C.

• S.O.C. Medium (6 ml)
Store S.O.C. Medium at 4°C or room temperature.

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