Invitrogen™

One Shot™ ccdB Survival™ 2 T1R Competent Cells

Catalog number:  A10460

Related applications:

Transformation

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Description

One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 109 transformants/µg plasmid DNA. The ccdB Survival™ 2 T1R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1R E. coli strain offers the following features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: ccdB Survival™ 2
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
Fast Growth: No
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Library Construction: No
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: One Shot™
Propagating ccdB Vectors: Suitable for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): Yes
Transformation Efficiency: > 1x10^9
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Species: E. coli
Format: One Shot
Improves Plasmid Quality: Yes

Contents & storage

Contains:
• ccdB Survival™2 T1R Competent Cells: 11 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.