Invitrogen™
One Shot™ ccdB Survival™ 2 T1R Competent Cells
Description
One Shot® ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 109 transformants/µg plasmid DNA. The ccdB Survival™ 2 T1R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1R E. coli strain offers the following features:• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)
Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Bacterial or Yeast Strain: | ccdB Survival™ 2 |
|---|---|
| Blue/White Screening: | Yes |
| Cloning Methylated DNA: | Yes |
| Cloning Unstable DNA: | Not Suitable for cloning unstable DNA |
| Competent Cell Type: | Chemically Competent |
| F'episome (to make ssDNA): | Lacks F' Episome |
| Fast Growth: | No |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Library Construction: | No |
| Preparing Unmethylated DNA: | Not Suitable for preparing unmethylated DNA |
| Product Line: | One Shot™ |
| Propagating ccdB Vectors: | Suitable for ccdB vector propagation |
| Reduces Recombination: | Yes |
| Shipping Condition: | Dry Ice |
| T1 Phage - Resistant (tonA): | Yes |
| Transformation Efficiency: | > 1x10^9 |
| Transformation Efficiency Level: | High Efficiency (> 10^9 cfu⁄µg) |
| Species: | E. coli |
| Format: | One Shot |
| Improves Plasmid Quality: | Yes |
Contents & storage
Contains:
• ccdB Survival™2 T1R Competent Cells: 11 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
• ccdB Survival™2 T1R Competent Cells: 11 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml
Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
