Invitrogen™

One Shot™ ccdB Survival™ 2 T1R Competent Cells

Catalog number: A10460
Invitrogen™

One Shot™ ccdB Survival™ 2 T1R Competent Cells

Catalog number: A10460
Catalog Number
A10460
Unit Size
11 x 50 µL/tube
Price (USD)
Price: 309.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1046011 x 50 µL/tube
Price: 309.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
One Shot™ ccdB Survival™ 2 T1R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway™ Vector Conversion System and for propagating Gateway™ destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 109 transformants/µg plasmid DNA. The ccdB Survival™ 2 T1R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1R E. coli strain offers the following features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Genotype: F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG fhuA::IS2
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cell
Contains F' Episome
Lacks F' Episome
Efficiency
High Efficiency (>10^9 cfu/µg)
Cell Type
Chemically Competent
Improves Plasmid Quality
Yes
Cloning Methylated DNA
Yes
Transformation Efficiency Level
High Efficiency (> 10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
ccdB Survival™ 2
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Suitable for ccdB vector propagation
Reduces Recombination
Yes
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
Yes
Species
E. coli
Format
One Shot
Product Line
One Shot™
Quantity
11 x 50 μL

Contents & Storage

Contains:
• ccdB Survival™2 T1R Competent Cells: 11 vials, 50 µl each
• pUC19 DNA (10 pg/µl): 1 vial, 50 µl
• S.O.C. medium: 1 bottle, 6 ml

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.

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