One Shot™ ccdB Survival™ 2 T1^R Chemically Competent Cells are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway™ Vector Conversion System and for propagating Gateway™ destination, donor, and supercoiled entry vectors. Transformation efficiency is >1 × 10⁹ transformants/µg plasmid DNA. The ccdB Survival™ 2 T1^R E. coli strain is derived from the TOP10 strain. The ccdB Survival™ 2 T1^R E. coli strain offers the following features:

• Resistance to the ccdB gene product
• Resistance to T1 and T5 phage (the tonA/fhuA phenotype)
• Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype)
• Reduced nonspecific recombination of cloned DNA (the recA1 phenotype)

Genotype: F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (Str^R) endA1 nupG fhuA::IS2