Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone.Read more
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Catalog number A10462
Price (USD)/ 10 µg
262.65
Online exclusive
284.00
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Price (USD)/ 10 µg
262.65
Online exclusive
284.00
Save 21.35 (8%)
Add to cart
Gateway™ pENTR™ 1A Dual Selection Vector
Catalog numberA10462
Price (USD)/ 10 µg
262.65
Online exclusive
284.00
Save 21.35 (8%)
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Add to cart
Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!
The Gateway™ entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only) • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli • pUC origin for high-copy replication and maintenance of the plasmid in E. coli • Kanamycin resistance gene for selection in E. coli • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection and o Chloramphenicol selection in E. coli • Kanamycin resistance gene for selection in E. coli
For Research Use Only. Not for use in diagnostic procedures.