Invitrogen™

Gateway™ pENTR™ 2B Dual Selection Vector

Catalog number: A10463
Invitrogen™

Gateway™ pENTR™ 2B Dual Selection Vector

Catalog number: A10463
Catalog Number
A10463
Unit Size
10 µg
Price (USD)
Price: 241.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1046310 µg
Price: 241.00
Your Price:
***
Product Overview
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Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!

The Gateway™ entry vectors (Table 1) offer the following:
• attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression
• Kozak consensus sequence for efficient translation initiation in eukaryotic systems
• Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only)
• rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli
• Kanamycin resistance gene for selection in E. coli
• The ccdB⁄chloramphenicol fusion gene located between the two attL sites for
o negative selection and
o Chloramphenicol selection in E. coli
• Kanamycin resistance gene for selection in E. coli
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Dual SelectionExpression Vector
Antibiotic Resistance Bacterial
Chloramphenicol (CmR), Kanamycin (KanR)
Cleavage
No Cleavage Site
Protein Tag
Untagged
Cloning Method
Gateway™
Vector
pENTR
Product Line
pENTR™

Contents & Storage

10 µg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0.
Store at -20°C

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