Invitrogen™

Competent Cell Sampler

Catalog number:  A10469

 Related applications:

Transformation

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Description

The Competent Cell Sampler kit contains a pair of four different strains of chemically competent E. coli cells in convenient One Shot formats. Each single-use vial of cells is ready to use, with no extra pipetting steps or freeze-thaw cycles which reduce transformation efficiency. Use this kit to evaluate which strains best suit the different types of DNA used in your lab.

One Shot Mach1 T1-phage resistant chemically competent E. coli cells have a faster doubling time than typical E. coli cells. As a result, Mach1 colonies are clearly visible within eight hours of plating your transformation on ampicillin selection plates. From an overnight colony, minipreps can be performed after four hours of growth.

Mach1 competent E. coli cells come in One Shot format (Cat. No. C862003) and our MultiShot StripWell and FlexPlate formats for medium and high-throughput applications (C869601 and C8681201).

One Shot OmniMAX2 T1-phage-resistant chemically competent E. coli cells are perfect for use in all cloning applications, including Gateway technology. They offer the highest transformation efficiency in a chemically competent One Shot format with 5 x 109 transformants with 1 µg of control DNA. Coupling the high transformation efficiency with the elimination of the mcrA, mrr, mcrBC, and hsdRMS restriction systems allows for the construction of more representative genomic libraries in a convenient chemically competent format.

OmniMAX 2 cells are offered in our One Shot format (C854003) and in our MultiShot FlexPlate format for medium to high throughput applications (C8581201).

OneShot Stbl3 chemically competent cells reduce the frequency of unwanted homologous recombination and are perfect for cloning unstable DNA such as lentiviral DNA containing long terminal repeats (LTRs). The DNA yield from Stbl3 cells is often >10-fold higher than standard cloning strains.

Stbl3 chemically competent cells are available in the One Shot format (C737303) and in the MultiShot StripWell and FlexPlate formats (C739601 and C7381201) for medium to high throughput applications.

One Shot TOP10 cells are perfect for a broad range of cloning techniques. They are often included with many of our cloning and expression kits like TOPO and Gateway cloning kits and complement our GeneArt High-Q Strings and Strings DNA fragments and libraries. One Shot TOP10 competent E. coli come with a transformation efficiency of 1 x 109 cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids.

TOP10 chemically competent cells are available in the One Shot format (C404003), in the MultiShot StripWell and FlexPlate formats (C409601 and C4081201) for medium to high throughput applications.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: Mach1™ , OmniMAX™2 , Stbl3™ , TOP10
Product Line: One Shot™
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Contains F' Episome
Fast Growth: Yes
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Library Construction: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 8 transformations
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Shipping Condition: Dry Ice

Contents & storage

Competent Cell Sampler contains two transformations each of four strains of chemically competent cells in One Shot® format. Store at -80°C.

Documents

Manuals & protocols