Competent Cell Sampler

Catalog number:  A10469

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Our Competent Cell Sampler includes two reactions of four chemically competent E. coli strains.
-One Shot® Mach1™ T1R, catalog #C862003, is the fastest-growing chemically competent strain currently available. Mach1™ T1R colonies are clearly visible within eight hours of plating your transformation. From an overnight colony, minipreps can be performed after four hours of growth.
Genotype: F– Φ80lacZΔM15 ΔlacX74 hsdR(rK–, mK +) ΔrecA1398 endA1 tonA
-One Shot® OmniMAX 2 T1R cells, catalog #C854003, are perfect for use in all cloning applications, including Gateway® technology. They offer the highest transformation efficiency of any chemically competent cell in the One Shot® packaging format.
Genotype: F´ {proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)} mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ(lacZYA-argF) U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD
-One Shot® Stbl3, catalog #C7373-03, reduce the frequency of unwanted homologous recombination and so are perfect for cloning unstable DNA.
Genotype: F– mcrB mrr hsdS20 (rB–, mB–) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 ?– leu mtl-1
-One Shot® TOP10 cells,C4040-03, are perfect for routine cloning and included in many TOPO® cloning and expression kits.
Genotype: F– mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endA1 nupG
Try them all to optimize transformations for your favorite clones
For Research Use Only. Not for use in diagnostic procedures.


Competent Cell Type: Chemically Competent
Species: E. coli
Format: One Shot
Transformation Efficiency: > 1x10^9
Bacterial or Yeast Strain: Mach1™ , OmniMAX™2 , Stbl3™ , TOP10
Product Line: One Shot™
Blue-White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Suitable for cloning unstable DNA
F' Episome (to make ssDNA): Contains F' Episome
Fast Growth: Yes
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Improves Plasmid Quality: Yes
Library Construction: Yes
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Size: 8 transformations
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
T1 Phage - Resistant (tonA): Yes
Shipping Condition: Dry Ice

Contents & storage

Competent Cell Sampler contains two transformations each of four strains of chemically competent cells in One Shot® format. Store at -80°C.


Manuals & protocols