Invitrogen™

APEX™ Antibody Labeling Kits

Catalog number: A10478
Invitrogen™

APEX™ Antibody Labeling Kits

Catalog number: A10478
Invitrogen APEX Antibody Labeling kits enable you to covalently label small quantities of IgG antibody (10–20 µg) with Alexa Fluor dyes or biotin, even in the presence of contaminants, stabilizing proteins, or amine-containing buffer (e.g., BSA, Tris, or glycine). APEX Antibody Labeling kits feature a resin tip that captures the IgG antibody, enabling the removal of contaminants prior to the labeling reaction with the conjugate of choice. After the application of the amine-reactive fluorophore to the IgG antibody bound on the resin, a fluorescent IgG conjugate is formed and subsequently eluted from the resin using elution buffer. Each kit contains everything needed for five labeling reactions. Working IgG antibody conjugate can be achieved in about 2.5 hours (with approximately 15 minutes of hands-on time).
 
Catalog Number
A10478
Unit Size
1 kit
Quantity
5 Reactions
Emission
455
Excitation
410
Label or Dye
Pacific Blue
Price (USD)
514.00
Your Price:
Availability
***
Full specifications
Quantity
Emission455
Excitation410
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorBlue
Labeling TargetAntibodies (IgG)
Label or DyePacific Blue
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
525
Excitation
490
Label or Dye
Alexa Fluor 488
Price (USD)
518.00
Your Price:
Availability
***
Full specifications
Quantity
Emission525
Excitation490
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorGreen
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 488
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
580
Excitation
555
Label or Dye
Alexa Fluor 555
Price (USD)
512.00
Your Price:
Availability
***
Full specifications
Quantity
Emission580
Excitation555
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorOrange
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 555
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
617
Excitation
590
Label or Dye
Alexa Fluor 594
Price (USD)
516.00
Your Price:
Availability
***
Full specifications
Quantity
Emission617
Excitation590
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorRed
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 594
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
665
Excitation
650
Label or Dye
Alexa Fluor 647
Price (USD)
524.00
Your Price:
Availability
***
Full specifications
Quantity
Emission665
Excitation650
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorFar-Red
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 647
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
603
Excitation
578
Label or Dye
Alexa Fluor 568
Price (USD)
510.00
Your Price:
Availability
***
Full specifications
Quantity
Emission603
Excitation578
Labeling Scale10-20 μg
Product LineAlexa Fluor
Chemical ReactivityAmine
ColorOrange
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 568
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Emission
not applicable
Excitation
not applicable
Label or Dye
Biotin-XX
Price (USD)
504.00
Your Price:
Availability
***
Full specifications
Quantity
Emissionnot applicable
Excitationnot applicable
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
Label TypeBiotin and other Haptens
Labeling TargetAntibodies (IgG)
Label or DyeBiotin-XX
Kit ContentsContains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Showing 7 of 7
Catalog NumberSpecificationsUnit SizeQuantityEmissionExcitationLabel or DyePrice (USD)AvailabilityQuantity
A10478Full specifications
1 kit5 Reactions455410Pacific Blue
514.00
Your Price:
***
Emission455
Excitation410
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorBlue
Labeling TargetAntibodies (IgG)
Label or DyePacific Blue
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10468Full specifications
1 kit5 Reactions525490Alexa Fluor 488
518.00
Your Price:
***
Emission525
Excitation490
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorGreen
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 488
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10470Full specifications
1 kit5 Reactions580555Alexa Fluor 555
512.00
Your Price:
***
Emission580
Excitation555
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorOrange
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 555
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10474Full specifications
1 kit5 Reactions617590Alexa Fluor 594
516.00
Your Price:
***
Emission617
Excitation590
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorRed
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 594
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10475Full specifications
1 kit5 Reactions665650Alexa Fluor 647
524.00
Your Price:
***
Emission665
Excitation650
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
ColorFar-Red
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 647
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10494Full specifications
1 kit5 Reactions603578Alexa Fluor 568
510.00
Your Price:
***
Emission603
Excitation578
Labeling Scale10-20 μg
Product LineAlexa Fluor
Chemical ReactivityAmine
ColorOrange
Label TypeAlexa Fluor Dyes
Labeling TargetAntibodies (IgG)
Label or DyeAlexa Fluor 568
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
A10495Full specifications
1 kit5 Reactionsnot applicablenot applicableBiotin-XX
504.00
Your Price:
***
Emissionnot applicable
Excitationnot applicable
Labeling Scale10-20 μg
Product LineAPEX
Chemical ReactivityAmine
Label TypeBiotin and other Haptens
Labeling TargetAntibodies (IgG)
Label or DyeBiotin-XX
Kit ContentsContains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Storage RequirementsStore at 2°C to 6°C, do not freeze, desiccate and protect from light.
Unit Size1 kit
Showing 7 of 7
Invitrogen APEX Antibody Labeling kits enable the covalent attachment of a fluorophore or biotin to small amounts of IgG antibody (∼10–20 µg), even in contaminated mixtures or solutions of biological samples. They are ideal for the efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Labeled antibodies are ready for use in imaging or flow cytometry applications in as little as 2.5 hours with little hands-on time.

Important features of APEX Antibody Labeling kits:
· Labeled antibodies typically ready to use in 2.5 hours (∼15 minutes hands-on time)
· Designed to label 10–20 µg of IgG
· Covalent attachment of Alexa Fluor dyes or biotin
· Compatible with contaminating proteins or stabilizers like BSA
· Choose from Alexa Fluor 488, 555, 568, 594, and 647 dyes, Pacific Blue dye, or Biotin-XX

Contaminating proteins or protein stabilizers are not a problem
Many IgG antibodies are often available only in small quantities and packaged with stabilizing proteins, such as BSA, or other contaminants that can interfere with the amine-reactive labeling reagents. The APEX Antibody Labeling kits avoid this issue by utilizing a solid-phase labeling technique that captures the IgG antibody on a specialized resin inside the APEX antibody labeling tip. Contaminants are simply eluted through the tip prior to the application of the amine-reactive label.

Better results and workflows with primary labeled antibodies
A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

Customization is possible
Can’t find what you’re looking for in our stocked list? We can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

For Research Use Only. Not for use in diagnostic procedures.

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