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CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Catalog number: A1048503

CTS™ OpTmizer™ T Cell Expansion SFM, bag format

Catalog number: A1048503
Catalog Number
also known as A10485-03
Unit Size
1,000 mL bag
Price (USD)
Price: 280.00
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
also known as A10485-03
1,000 mL bag
Price: 280.00
Your Price:

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Product Overview
Citations & References
Additional Information
Gibco™ OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free and xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bag) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function as T-cells cultured in conventional serum supplemented medium
• Consistent performance in supporting T-cell expansion
• Supports high density cultures in static and WAVE Bioreactor™ cultures
• Be ready for clinical research with this cGmP manufactured formula and support documentation

Versatile T Cell Culture Media
OpTmizer™ CTS™ T-Cell Expansion SFM is designed to give results equivalent to conventional serum-supplemented media. However, if desired, 2% heat-inactivated human serum (Cat. No. 34005-100) may be added to the medium to enhance viability and expansion. The use of serum, and the amount required, should be determined empirically depending on the specific T-cell culture application.

Ready for T Cell Expansion
OpTmizer™ CTS™ T-Cell Expansion SFM is designed for the expansion of human T-cells permitting CD3+ densities of >3x106 cells/mL in static conditions and >2x107 cells/mL in WAVE bags. Results may vary depending on the T-cell subset (e.g., Ag-specific cells). Conditions for specific T-cell culture applications should be determined empirically.

Stability of Complete Media
Complete OpTmizer™ CTS™ T-Cell Expansion SFM (basal, supplement, and L-glutamine) is stable for 4 weeks when stored in the dark at 2–8°C.

Simplify Your Transition into Clinical Research
The Gibco™ Cell Therapy Systems (CTS™) brand offers a broad array of products for use in cell therapy applications, including media, reagents, growth factors, enzymes, cell selection/expansion reagents, and devices. Gibco™ CTS™ products deliver:
• Reduced burden in qualifying reagents
• Appropriate certificates of analyses and origin included
• Convenient access to our Drug Master File (DMF)


Culture Environment
Cell Type
T Cells
Chemically-defined, Serum-free, Xeno-free
Culture Type
Suspension Cell Culture
Product Type
Cell Expansion Serum Free Medium (SFM)
Serum Level
With Additives
HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate
Without Additives
No Glutamine
Endotoxin Level
Very Low
Included Antibiotics
No antibiotics
Inorganic Salts
Calcium, Magnesium
Purity or Quality Grade
Research Grade
1 bag

Contents & Storage

OpTmizer™ T-Cell Expansion Basal Medium (1 x L media bag): Store at 2–8°C. Protect from light.

OpTmizer™ T-Cell Expansion Supplement (1 x 26 mL): Store in the dark at 2–8°C.