Invitrogen™

APEX™ Antibody Labeling Kits

Name: APEX™ Antibody Labeling Kits
SKU: A10494

Catalog number: A10494

Related applications: Labeling Chemistry

Protein Labeling & Crosslinking

Technical SupportCustomer Service

Invitrogen™

APEX™ Antibody Labeling Kits

Catalog number: A10494

Related applications: Labeling Chemistry

Protein Labeling & Crosslinking

Technical SupportCustomer Service

Invitrogen APEX Antibody Labeling kits enable you to covalently label small quantities of IgG antibody (10–20 µg) with Alexa Fluor dyes or biotin, even in the presence of contaminants, stabilizing proteins, or amine-containing buffer (e.g., BSA, Tris, or glycine). APEX Antibody Labeling kits feature a resin tip that captures the IgG antibody, enabling the removal of contaminants prior to the labeling reaction with the conjugate of choice. After the application of the amine-reactive fluorophore to the IgG antibody bound on the resin, a fluorescent IgG conjugate is formed and subsequently eluted from the resin using elution buffer. Each kit contains everything needed for five labeling reactions. Working IgG antibody conjugate can be achieved in about 2.5 hours (with approximately 15 minutes of hands-on time).

Catalog Number

A10494

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

578/603

Label or Dye

Alexa Fluor™ 568

Price (USD)

552.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	578/603
Labeling Scale	10 to 20 μg
Product Line	Alexa Fluor™
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 568
Label Type	Alexa Fluor Dyes
Color	Orange
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10468

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

490/525

Label or Dye

Alexa Fluor™ 488

Price (USD)

560.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	• Reactive Fluorescent Label, 5 vials • APEX antibody labeling tips, 5 Ea. • Wash Buffer, 1.8 mL • Dimethylsulfoxide (DMSO), 100 μL • Labeling Buffer, 100 μL • Neutralization Buffer, 100 μL • Elution Buffer, 1 mL • Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	490/525
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 488
Label Type	Alexa Fluor Dyes
Color	Green
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10470

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

555/580

Label or Dye

Alexa Fluor™ 555

Price (USD)

554.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	555/580
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 555
Label Type	Alexa Fluor Dyes
Color	Orange
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10474

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

590/617

Label or Dye

Alexa Fluor™ 594

Price (USD)

558.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	590/617
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 594
Label Type	Alexa Fluor Dyes
Color	Red
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10475

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

650/665

Label or Dye

Alexa Fluor™ 647

Price (USD)

566.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	650/665
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 647
Label Type	Alexa Fluor Dyes
Color	Far-Red
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10478

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

410/455

Label or Dye

Pacific Blue™

Price (USD)

556.00

Your Price:

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	410/455
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Pacific Blue™
Color	Blue
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Catalog Number

A10495

Unit Size

1 kit

Quantity

5 Reactions

Excitation/Emission

Label or Dye

Biotin-XX

Price (USD)

546.00 Contact Us ›

Availability

***

Full specifications

Quantity

Kit Contents	Contains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Biotin-XX
Label Type	Biotin & Other Haptens
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Showing 7 of 7

Catalog Number Specifications Unit Size Quantity Excitation/Emission Label or Dye Price (USD) Availability Quantity

A10494

Full specifications

1 kit

5 Reactions

578/603

Alexa Fluor™ 568

552.00

Your Price:

***

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	578/603
Labeling Scale	10 to 20 μg
Product Line	Alexa Fluor™
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 568
Label Type	Alexa Fluor Dyes
Color	Orange
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10468

Full specifications

1 kit

5 Reactions

490/525

Alexa Fluor™ 488

560.00

Your Price:

***

Kit Contents	• Reactive Fluorescent Label, 5 vials • APEX antibody labeling tips, 5 Ea. • Wash Buffer, 1.8 mL • Dimethylsulfoxide (DMSO), 100 μL • Labeling Buffer, 100 μL • Neutralization Buffer, 100 μL • Elution Buffer, 1 mL • Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	490/525
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 488
Label Type	Alexa Fluor Dyes
Color	Green
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10470

Full specifications

1 kit

5 Reactions

555/580

Alexa Fluor™ 555

554.00

Your Price:

***

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	555/580
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 555
Label Type	Alexa Fluor Dyes
Color	Orange
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10474

Full specifications

1 kit

5 Reactions

590/617

Alexa Fluor™ 594

558.00

Your Price:

***

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	590/617
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 594
Label Type	Alexa Fluor Dyes
Color	Red
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10475

Full specifications

1 kit

5 Reactions

650/665

Alexa Fluor™ 647

566.00

Your Price:

***

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	650/665
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Alexa Fluor™ 647
Label Type	Alexa Fluor Dyes
Color	Far-Red
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10478

Full specifications

1 kit

5 Reactions

410/455

Pacific Blue™

556.00

Your Price:

***

Kit Contents	Contains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Excitation/Emission	410/455
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Pacific Blue™
Color	Blue
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

A10495

Full specifications

1 kit

5 Reactions

Biotin-XX

546.00 Contact Us ›

***

Kit Contents	Contains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity	5 Reactions
Labeling Scale	10 to 20 μg
Product Line	APEX
Chemical Reactivity	Amine
Label or Dye	Biotin-XX
Label Type	Biotin & Other Haptens
Labeling Target	Antibodies (IgG)
Unit Size	1 kit

Showing 7 of 7

Invitrogen APEX Antibody Labeling kits enable the covalent attachment of a fluorophore or biotin to small amounts of IgG antibody (∼10–20 µg), even in contaminated mixtures or solutions of biological samples. They are ideal for the efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Labeled antibodies are ready for use in imaging or flow cytometry applications in as little as 2.5 hours with little hands-on time.

Important features of APEX Antibody Labeling kits:
· Labeled antibodies typically ready to use in 2.5 hours (∼15 minutes hands-on time)
· Designed to label 10–20 µg of IgG
· Covalent attachment of Alexa Fluor dyes or biotin
· Compatible with contaminating proteins or stabilizers like BSA
· Choose from Alexa Fluor 488, 555, 568, 594, and 647 dyes, Pacific Blue dye, or Biotin-XX

Contaminating proteins or protein stabilizers are not a problem
Many IgG antibodies are often available only in small quantities and packaged with stabilizing proteins, such as BSA, or other contaminants that can interfere with the amine-reactive labeling reagents. The APEX Antibody Labeling kits avoid this issue by utilizing a solid-phase labeling technique that captures the IgG antibody on a specialized resin inside the APEX antibody labeling tip. Contaminants are simply eluted through the tip prior to the application of the amine-reactive label.

Better results and workflows with primary labeled antibodies
A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

Customization is possible
Can’t find what you’re looking for in our stocked list? We can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.