Invitrogen™

APEX™ Antibody Labeling Kits

Catalog number: A10495
Invitrogen™

APEX™ Antibody Labeling Kits

Catalog number: A10495
Invitrogen APEX Antibody Labeling kits enable you to covalently label small quantities of IgG antibody (10–20 µg) with Alexa Fluor dyes or biotin, even in the presence of contaminants, stabilizing proteins, or amine-containing buffer (e.g., BSA, Tris, or glycine). APEX Antibody Labeling kits feature a resin tip that captures the IgG antibody, enabling the removal of contaminants prior to the labeling reaction with the conjugate of choice. After the application of the amine-reactive fluorophore to the IgG antibody bound on the resin, a fluorescent IgG conjugate is formed and subsequently eluted from the resin using elution buffer. Each kit contains everything needed for five labeling reactions. Working IgG antibody conjugate can be achieved in about 2.5 hours (with approximately 15 minutes of hands-on time).
 
Catalog Number
A10495
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
Label or Dye
Biotin-XX
Price (USD)
Availability
***
Full specifications
Quantity
-
Kit ContentsContains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeBiotin-XX
Label TypeBiotin & Other Haptens
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
490/525
Label or Dye
Alexa Fluor™ 488
Price (USD)
560.00
Your Price:
Availability
***
Full specifications
Quantity
Kit Contents• Reactive Fluorescent Label, 5 vials
• APEX antibody labeling tips, 5 Ea.
• Wash Buffer, 1.8 mL
• Dimethylsulfoxide (DMSO), 100 μL
• Labeling Buffer, 100 μL
• Neutralization Buffer, 100 μL
• Elution Buffer, 1 mL
• Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission490/525
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
555/580
Label or Dye
Alexa Fluor™ 555
Price (USD)
554.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission555/580
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 555
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
590/617
Label or Dye
Alexa Fluor™ 594
Price (USD)
558.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission590/617
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
650/665
Label or Dye
Alexa Fluor™ 647
Price (USD)
566.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission650/665
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-Red
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
410/455
Label or Dye
Pacific Blue™
Price (USD)
556.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission410/455
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyePacific Blue™
ColorBlue
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Quantity
5 Reactions
Excitation/Emission
578/603
Label or Dye
Alexa Fluor™ 568
Price (USD)
552.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission578/603
Labeling Scale10 to 20 μg
Product LineAlexa Fluor™
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 568
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Showing 7 of 7
Catalog NumberSpecificationsUnit SizeQuantityExcitation/EmissionLabel or DyePrice (USD)AvailabilityQuantity
A10495Full specifications
1 kit5 ReactionsBiotin-XX***-
Kit ContentsContains Reactive Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeBiotin-XX
Label TypeBiotin & Other Haptens
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10468Full specifications
1 kit5 Reactions490/525Alexa Fluor™ 488
560.00
Your Price:
***
Kit Contents• Reactive Fluorescent Label, 5 vials
• APEX antibody labeling tips, 5 Ea.
• Wash Buffer, 1.8 mL
• Dimethylsulfoxide (DMSO), 100 μL
• Labeling Buffer, 100 μL
• Neutralization Buffer, 100 μL
• Elution Buffer, 1 mL
• Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission490/525
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 488
Label TypeAlexa Fluor Dyes
ColorGreen
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10470Full specifications
1 kit5 Reactions555/580Alexa Fluor™ 555
554.00
Your Price:
***
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission555/580
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 555
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10474Full specifications
1 kit5 Reactions590/617Alexa Fluor™ 594
558.00
Your Price:
***
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission590/617
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 594
Label TypeAlexa Fluor Dyes
ColorRed
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10475Full specifications
1 kit5 Reactions650/665Alexa Fluor™ 647
566.00
Your Price:
***
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission650/665
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 647
Label TypeAlexa Fluor Dyes
ColorFar-Red
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10478Full specifications
1 kit5 Reactions410/455Pacific Blue™
556.00
Your Price:
***
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission410/455
Labeling Scale10 to 20 μg
Product LineAPEX
Chemical ReactivityAmine
Label or DyePacific Blue™
ColorBlue
Labeling TargetAntibodies (IgG)
Unit Size1 kit
A10494Full specifications
1 kit5 Reactions578/603Alexa Fluor™ 568
552.00
Your Price:
***
Kit ContentsContains Reactive Fluorescent Label, 5 vials; APEX antibody labeling tips, 5 Ea.; Wash Buffer, 1.8 mL; Dimethylsulfoxide (DMSO), 100 μL; Labeling Buffer, 100 μL; Neutralization Buffer, 100 μL; Elution Buffer, 1 mL; Elution Syringe, 1 Ea.
Quantity5 Reactions
Excitation/Emission578/603
Labeling Scale10 to 20 μg
Product LineAlexa Fluor™
Chemical ReactivityAmine
Label or DyeAlexa Fluor™ 568
Label TypeAlexa Fluor Dyes
ColorOrange
Labeling TargetAntibodies (IgG)
Unit Size1 kit
Showing 7 of 7
Invitrogen APEX Antibody Labeling kits enable the covalent attachment of a fluorophore or biotin to small amounts of IgG antibody (∼10–20 µg), even in contaminated mixtures or solutions of biological samples. They are ideal for the efficient labeling of antibodies in serum, ascites fluid, or hybridoma suspensions. Labeled antibodies are ready for use in imaging or flow cytometry applications in as little as 2.5 hours with little hands-on time.

Important features of APEX Antibody Labeling kits:
· Labeled antibodies typically ready to use in 2.5 hours (∼15 minutes hands-on time)
· Designed to label 10–20 µg of IgG
· Covalent attachment of Alexa Fluor dyes or biotin
· Compatible with contaminating proteins or stabilizers like BSA
· Choose from Alexa Fluor 488, 555, 568, 594, and 647 dyes, Pacific Blue dye, or Biotin-XX

Contaminating proteins or protein stabilizers are not a problem
Many IgG antibodies are often available only in small quantities and packaged with stabilizing proteins, such as BSA, or other contaminants that can interfere with the amine-reactive labeling reagents. The APEX Antibody Labeling kits avoid this issue by utilizing a solid-phase labeling technique that captures the IgG antibody on a specialized resin inside the APEX antibody labeling tip. Contaminants are simply eluted through the tip prior to the application of the amine-reactive label.

Better results and workflows with primary labeled antibodies
A primary antibody directly labeled with a fluorophore often produces lower background fluorescence and less nonspecific binding. Further, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

Customization is possible
Can’t find what you’re looking for in our stocked list? We can prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

Contents & Storage

Store at 2°C to 6°C, do not freeze, desiccate and protect from light.

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