This product has been superseded by the RiboMinus™ Eukaryote System v2 and Low Input RiboMinus™ Eukaryote Kit v2 and will be discontinued in the near future.
Invitrogen™

RiboMinus™ Eukaryote Kit for RNA-Seq

Catalog number:  A1083708

Related applications:

DNA & RNA Purification & Analysis

| Sequencing

| Sequencing

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Description

The RiboMinus™ Eukaryote Kit for RNA-Seq provides truly complete transcriptome isolation for next generation sequencing analysis by offering a method for selective depletion of up to 99% of the 5S, 5.8S, 18S and 28S ribosomal RNA from total RNA versus poly-A or cap binding methods, which can introduce bias and select for only part of the transcriptome.

The RiboMinus™ Eukaryote Kit for RNA-Seq Offers:

• A simple, yet selective depletion procedure that uses a sequence-specific probe to capture rRNA
• Rapid and efficient removal of rRNA-probe complex using magnetic bead technology

Three Steps to an rRNA-free Preparation Suitable for Transcriptome Analysis
The removal of large ribosomal RNA using the RiboMinus™ kit is a reliable and robust three-step procedure. Hybridize the RNA sample to the RiboMinus™ Probe in a water bath or heat block, then remove probe-rRNA complexes using the RiboMinus™ magnetic beads included in the kit. Concentrate the depleted RNA preparation by ethanol precipitation, or by using the RiboMinus™ Concentration Module.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: Transcriptome RNA
For Use With (Application): Microarray Analysis, Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Purification Target: Transcriptome RNA
Sample Type: Total RNA
Isolation Technology: Magnetic Bead
Quantity: 1 each
Yield: 1 µg
High-throughput Compatibility: High-throughput Compatible
Sequencing Type: Transcriptome Sequencing
Shipping Condition: Wet Ice
Starting Material Cell No.: 2-10 µg total RNA
Starting Material Amount: 2-10 µg Total RNA
Starting Material Volume: 20 µl
System Type: RiboMinus
Product Line: RiboMinus™

Contents & storage

Each kit contains:
• 6 ml RiboMinus™ Magnetic Beads
• 80 µl RiboMinus™ Eukaryote Probe (15 pmol/µl)
• 10 ml Hybridization Buffer
• 12 ml DEPC-treated (RNase-free) water
Store at 4°C.