Invitrogen™

RiboMinus™ Plant Kit for RNA-Seq

Catalog number:  A1083808

Related applications:

RNA Extraction

| RNA Sequencing

| SOLiD® Next-Generation Sequencing

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Description

RiboMinus™ Plant Kit for RNA-Seq is the complete solution for transcriptome isolation and enrichment of the true whole transcriptome through selective depletion of ribosomal RNA in plant species. This kit offers additional plastid depletion probes designed to target chloroplast rRNA not offered in the RiboMinus™ Eukaryote Kit for RNA-Seq critical for enhanced depletion of ribosomal RNA in plants. The RiboMinus™ Plant Kit for RNA-Seq contains all the components necessary to deplete ribosomal RNA from plant species. This improvement allows for up to 99.8% depletion of the major rRNA peaks in many plant species. The RiboMinus™ Plant Kit for RNA-Seq is recommended for wheat, maize, rice, barley, soybean, sugar cane, sorghum, potato, cassava, Arabidopsis, tomato, tobacco, poplar, legume, and moss. Additional plant species can be bioinformatically assessed by contacting Invitrogen.

Working with something other than human, mouse, or plant? Contact us with your species and sequence. Simply include your FASTA sequence in *.txt file format for the ribosomal sequences you would like to verify, with the subject line titled 'RiboMinus for (your organism here)', and we'll work with you to find the right probe for your RiboMinus® rRNA depletion.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Final Product Type: Transcriptome RNA
For Use With (Application): Next-Generation Sequencing, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Sequencing, Northern Blotting, cDNA Library Construction
Purification Target: Transcriptome RNA
Sample Type: Total RNA (Plant)
Isolation Technology: Magnetic Bead
Yield: 1 µg
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Sequencing Type: Transcriptome Sequencing
Shipping Condition: Wet Ice
Starting Material Amount: 2-10 µg Total RNA
Starting Material Volume: 20 µl
System Type: RiboMinus
Product Line: RiboMinus™

Contents & storage

Ribominus™ Plant Probe 88 µL; Hybridization Buffer 11 mL; Rnase Free Water 13.2 mL; RiboMinus™ Capture Beads 6 mL, User Manual