Jump-In™ Fast Gateway® System

Catalog number: A10893

Invitrogen™  Related applications: Protein Expression

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The Jump-In™ Fast Gateway® System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway® System consists of:

• The Jump-In™ Fast Gateway® Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway® Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: None (Promoterless)
Key Function: Stable Cell Line Development, Targeted Integration, Clone your own Promoter
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Hygromycin
Vector Type: pJTI, Jump-In Vectors
Expression: Mammalian
Product Size: 20 reactions
High Throughput Compatibility: High Throughput-Compatible
Selection Marker (Eukaryotic): HygroR
Constitutive or Inducible System: Constitutive

Contents & storage

Jump-In™ Fast Gateway® System is composed of the Jump-In™ Fast Gateway® Core Kit and the MultiSite Gateway® Pro Plus Kit (includes One Shot® Mach1™ T1R Chemically Competent Cells, and LR Clonase® II Plus and BP Clonase® II enzymes).

This kit has components that can also be sold separately.

Store cells at -80°C.
Store Clonase® enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.


Manuals & protocols