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Jump-In™ Fast Gateway™ SystemThe Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integrationRead more
Catalog number A10893
Price (USD)/ 20 reactions
6,900.00
-
Price (USD)/ 20 reactions
6,900.00
Jump-In™ Fast Gateway™ System
Catalog numberA10893
Price (USD)/ 20 reactions
6,900.00
-
The Jump-In™ Fast Gateway™ System is intended for rapid engineering of mammalian cell lines through the stable and irreversible integration of your gene of interest (GOI) at specific genomic loci (pseudo attP sites). Following stable integration, only 5-10 clones need to be analyzed to identify clones with high expression levels.
The Jump-In™ Fast Gateway™ System consists of:
• The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.
The Jump-In™ Fast Gateway™ System consists of:
• The Jump-In™ Fast Gateway™ Core Kit containing the promoter-less pJTI™ Fast DEST expression vector and a vector for transient expression of the PhiC31 integrase.
• The MultiSite Gateway™ Pro Plus kit for cloning of up to 4 gene fragments into the promoter-less pJTI™ Fast DEST expression vector.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodGateway™
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
Cell TypeMammalian
PromoterNone (Promoterless)
VectorJump-In Vectors, pJTI
Expression SystemMammalian
For Use With (Application)Protein Expression
High-throughput CompatibilityHigh-throughput Compatible
Key FunctionsStable Cell Line Development, Targeted Integration, Clone your own Promoter
No. of Reactions20 reactions
Product LineJump-In™
Product TypeCloning Kit
Protein TagUntagged
Selection Agent (Eukaryotic)Hygromycin
Selection Marker EukaryoticHygroR
Unit Size20 reactions
Contents & Storage
Jump-In™ Fast Gateway™ System is composed of the Jump-In™ Fast Gateway™ Core Kit and the MultiSite Gateway™ Pro Plus Kit (includes One Shot™ Mach1™ T1R Chemically Competent Cells, and LR Clonase™ II Plus and BP Clonase™ II enzymes).
This kit has components that can also be sold separately.
Store cells at -80°C.
Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.
This kit has components that can also be sold separately.
Store cells at -80°C.
Store Clonase™ enzymes at -20°C for up to 6 months, or at -80°C for long-term storage.
Store all other components, including vectors, at -20°C.