Jump-In™TI™ Platform Kit

Catalog number: A10897

Invitrogen™  Related applications: Protein Expression | Stem Cell Engineering & Reprogramming

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The Jump-In™TI™ Platform Kit enables generation of a Jump-In™TI™ cell line. A Jump-In™TI™ cell line is created by co-transfecting one of three vectors containing the R4 attP target site with the transiently expressed vector for the PhiC31 integrase into your mammalian cell line.
Generating a Jump-In™TI™ cell line is the first step in creating a stable, targeted, and irreversible mammalian cell line expressing your gene of interest. For targeted and irreversible integration of your gene of interest into your Jump-In™TI™ cell line, use the Jump-In™TI™ Gateway® Vector Kit.
This kit is also a component of the Jump-In™TI™ Gateway® System.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: None (Promoterless)
Key Function: Stable Cell Line Development, Targeted Integration, Clone your own Promoter
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Geneticin® (G-418), Zeocin™, Blasticidin
Vector Type: pJTI, Jump-In Vectors
Expression: Mammalian
Product Size: 1 kit
High Throughput Compatibility: High Throughput-Compatible
Selection Marker (Eukaryotic): NeoR, ZeoR, BsdR

Contents & storage

The Jump-In™TI™ Platform Kit is composed of three vectors:
pJTI™⁄Bsd Vector (500 ng⁄µl; total: 20 µl),
pJTI™⁄Neo Vector (500 ng⁄µl; total: 20 µl),
pJTI™⁄Zeo Vector (500 ng⁄µl; total 20 µl),
pJTI™ PhiC31 Int Vector (0.5 µg⁄µl; total 20 µl)

Store vectors at -20°C.


Manuals & protocols