Blasticidin S HCl (10 mg/mL)

Catalog number: A1113903

Gibco™  Related applications: Insect Cell Culture | Mammalian Cell Culture | Transfection | Transformation

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Description

Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome.

Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 1–20 µg/mL depending on the cell line. This product is provided as a solution at 10 mg/mL in 20 mM HEPES (pH 7.2–7.5) and is supplied in 10 vials each containing 1 mL.

In addition, Blasticidin S is used as a bacterial selection antibiotic in the concentration range of 50–100 µg/mL. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD or bsr. These deaminases convert Blasticidin S to a nontoxic derivative. To maintain the activity of Blasticidin S for selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0.

Other Choices and More Information
We offer a wide range of antibiotics and antimycotics in both powder and liquid formats.

See the complete list, or find products for:
Contamination control
Eukaryotic and bacterial selection

See recommendations for working concentrations for selection antibiotics.

Learn more about the use of antibiotics and antimycotics in cell culture, and review guidelines for decontaminating cultures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Validated Application: Bacterial Selection, Eukaryotic Selection⁄Stable Cell Line Generation
Agent: Blasticidin
Form: Liquid
Reagent Type: Antibiotic (Selective)
Concentration: 10 mg⁄ml
Formula Weight: 458.9 g⁄mole
Product Size: 10 x 1 mL
Quantity: 10 vials
Sterility: Sterile
Shipping Condition: Dry Ice

Contents & storage

Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Frozen
Shelf life: 9 months from date of manufacture

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