Invitrogen™

ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

Catalog number: A11144
Invitrogen™

ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit

Catalog number: A11144
Catalog Number
A11144
Unit Size
20 reactions
Price (USD)
3,440.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1114420 reactions
3,440.00
Your Price:
***
Product Overview
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The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ HiPerform™ tetracycline-regulated lentiviral expression vector, pLenti6.3⁄TO⁄V5-DEST™ for easy recombination-based cloning and lentiviral-based, regulated, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄ TO⁄ V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.

Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway™ technology
• Stable, long-term, tetracycline-regulated expression
• Enhanced protein expression, up to 4-fold or greater, compared to traditional lentiviral expression systems

Key Features
• WPRE from the woodchuck hepatitis virus, increases transgene expression and cPPT from the HIV-1 integrase gene, increases the copy number of lentivirus integrating into the host genome, thus increasing viral titer. WPRE and cPPT together produce at least a four-fold increase in protein expression in most cell types, compared to other vectors that do not contain these elements.
• Hybrid promoter consisting of the human cytomegalovirus (CMV) promoter and two tetracycline operator 2 (TetO2) sites for high-level, regulated expression of the target gene
• Blasticidin selection marker for stable selection under control of SV40 promoter

Kit includes
• pLenti6.3⁄TO⁄V5-DEST™ vector
• pLenti3.3⁄TR vector
• pLenti6.3⁄TO⁄V5-GW⁄lacZ plasmid
• Tetracycline
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat # C737303)
• pENTRTM Gus positive control plasmid.

For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Constitutive or Inducible System
Inducible
Delivery Type
Lentiviral
Inducing Agent
Doxycycline, Tetracycline
Promoter
CMV/TO
Product Type
Vector Kit
Selection Agent (Eukaryotic)
Blasticidin
Antibiotic Resistance Bacterial
Ampicillin (AmpR), Blasticidin (BsdR)
Cleavage
No Cleavage Site
Gateway Enzyme
LR Clonase™ II Plus, LR Clonase™ II
PCR Enzyme
Proof-Reading Polymerase
Protein Tag Position (to your gene)
C-terminal
Replication Origin
pUC Origin
Selection Marker Promoter
PGK Promoter
Selection Marker Eukaryotic
BsdR
Special Elements
WPRE, cPPT
Protein Tag
V5 Epitope Tag
Cloning Method
Gateway™
Vector
pLenti
Product Line
Gateway™, HiPerform™, T-REx™, ViraPower™
For Use With (Application)
Viral Expression

Contents & Storage

Contents
The ViraPower™ HiPerform™ T-REx™ Gateway™ Vector Kit includes the following components:
• 6 ug of pLenti6.3⁄TO⁄V5-DEST™ vector (40 µL of vector at 150 ng⁄µL in TE Buffer, pH 8.0)
• 20 ug of pLenti3.3⁄TR vector (40 µL of vector at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 10 ug of pLenti6.3⁄TO⁄V5-GW⁄lacZ plasmid (20 µL of plasmid at 0.5 µg⁄µL in TE Buffer, pH 8.0)
• 1 ml of Tetracycline (10 mg⁄mL in water)
• One Shot™ Stbl3™ Chemically Competent E. coli (Cat # C737303): 21 X 50 µL of Stbl3™ Cells, 6 mL of S.O.C. Medium and 50 µL of pUC19 control DNA (10 pg⁄µL).
• pENTR™ Gus positive control plasmid.

Storage
• Vectors: -20°C
• Tetracycline: -20°C (protected from light)
• One Shot™ Stbl3™ Chemically Competent E. coli: -80°C
• pENTR™ Gus positive Control: -20°C

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