PichiaPink™ Media Kit

Catalog number: A11156

Invitrogen™  Related applications: Protein Expression

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Description

The PichiaPink™ Media Kit is a set of convenient prepackaged media pouches for use with the PichiaPink™ Yeast Expression System. The PichiaPink™ Media Kit contains media needed to grow Pichia pastoris strains from frozen stocks to cultures ready for transformation and selection. The PichiaPink™ Media Kit is an easy way get started using Pichia pastoris for protein production. Use the PichiaPink™ Media Kit to refill your PichiaPink™ Yeast Expression System Kits.

The PichiaPink™ Media Kit comes with prepackaged pouches for the following solutions. Each pouch makes 1 liter of solution. Media pouches should be kept dry and stored at room temperature.

MediaUsePouches
YP+agarFor plates to streak out colonies2 pouches
YPFor liquid cultures2 pouches
PAD (Pichia Adenine Dropout)+agarFor plates to grow PichiaPink™ recombinants2 pouches
YP+sorbital (YPS)For recovering PichiaPink™ transformants2 pouches
DextroseFor adding to YP and YP+agar1 pouch

Why Choose the PichiaPink™ Yeast Expression System?
The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris. Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling. For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2). The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales.

For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links
• Learn more about the PichiaPink™ Yeast Expression System.
• Learn more about our other protein expression systems.

References

1. Cereghino JL, Cregg JM. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev. 2000 Jan;24(1):45-66. [PubMed]
2. Cereghino GP, Cereghino JL, Ilgen C, Cregg JM. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr Opin Biotechnol. 2002 Aug;13(4):329-32. [PubMed]
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Compatible Products: A11150, A11151, A11152, A11153
Target Organism Class: P. pastoris
Bacterial or Yeast Strain: PichiaPink™
Form: Powder
Final Product: Agar Plates, Liquid Medium
Preparation Method: Autoclave
Product Size: 1 kit
Other Additives: Dextrose
Shipping Condition: Room Temperature

Contents & storage

Contains pouches sufficient to make 4 basic media:

2 pouches PAD agar, 2 pouches YPDS, 2 pouches YPD, 2 pouches YPD agar, 1 pouch Dextrose


Store at room temperature

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Manuals & protocols

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