CD OptiCHO™ AGT™ Medium

Description

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

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Specifications

Cell Line:	CHO
Classification:	Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Comments:	Medium does not support wild type DG44 cells
Culture Environment:	CO2
Culture Type:	Suspension Cell Culture
Endotoxin Level:	Low
Form:	Powder (AGT)
Format:	Securitainer Capped Vial
Glucose:	High Glucose
Glutamine:	No Glutamine
Included Antibiotics:	No antibiotics
Inorganic Salts:	Magnesium, Sodium Phosphate
Phenol Red Indicator:	No Phenol Red
Product Line:	AGT™, Gibco®
Product Size:	1 L
Purity or Quality Grade:	Certified
Reconstituted Volume:	1 L
Serum Level:	Serum-Free
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Sodium Pyruvate Additive:	Sodium Pyruvate
Sterility:	Nonsterile
Shipping Condition:	Wet Ice

Contents & storage