Invitrogen™

MembranePro™ Functional Protein Expression Kit

Catalog number: A11667
Have Questions?
Invitrogen™

MembranePro™ Functional Protein Expression Kit

Catalog number: A11667
Have Questions?
Catalog Number
A11667
Unit Size
10 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1166710 reactions
-
Product Overview
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For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO™ vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Type
MembranePro Functional Protein Expression Kit
Delivery Type
Virus-Like Particles (VLPs)
Quantity
1 Kit
Selection Agent (Eukaryotic)
Blasticidin
Cell Line
293FT
Expression Mechanism
Cell-Based Expression
High-throughput Compatibility
High-throughput Compatible
Selection Marker Eukaryotic
BsdR
Expression System
Mammalian
Expressed Protein Type
Membrane Protein
Product Line
MembranePro™
For Use With (Application)
Membrane Protein Expression
Promoter
EF-1α

Contents & Storage

The kit contains:
•pEF6 V5-His TOPO™ TA Vector Kit for at least 10 TOPO™ cloning reactions (Store at -20°C)
•3 x10^6 293FT Cells (Store in liquid nitrogen)
•1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
•1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
•3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
•Manuals

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