For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:
• Proteins are displayed on human (or other mammalian) cell membranes • Cellular quality control and mammalian posttranslational processing helps produce functional protein • Proteins bud off as lipoparticles and are easily collected from the culture medium • Lipoparticles are enriched with expressed receptors
MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:
1. Clone your gene of interest using a TOPO® vector 2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells 3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix 4. Store lipoparticles at -80 C or proceed to downstream assays
MembranePro™ products are offered in two configurations – as an expression kit (TOPO® vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.
For Research Use Only. Not for use in diagnostic procedures.
For Use With (Application)
Membrane Protein Expression
Selection Agent (Eukaryotic)
Selection Marker Eukaryotic
Expressed Protein Type
Virus-Like Particles (VLPs)
Contents & Storage
The kit contains: •pEF6 V5-His TOPO™ TA Vector Kit for at least 10 TOPO™ cloning reactions (Store at -20°C) •3 x10^6 293FT Cells (Store in liquid nitrogen) •1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature) •1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C) •3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C) •Manuals