Invitrogen™

MembranePro™ Functional Protein Expression Kit

Catalog number: A11667
Invitrogen™

MembranePro™ Functional Protein Expression Kit

Catalog number: A11667
Catalog Number
A11667
Unit Size
10 reactions
Price (USD)
2,670.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A1166710 reactions
2,670.00
Your Price:
***
Product Overview
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Recommendations
For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO™ vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO™ vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Delivery Type
Virus-Like Particles (VLPs)
Quantity
1 Kit
Selection Agent (Eukaryotic)
Blasticidin
Cell Line
293FT
Expression Mechanism
Cell-Based Expression
High-throughput Compatibility
High-throughput Compatible
Selection Marker Eukaryotic
BsdR
Expression System
Mammalian
Expressed Protein Type
Membrane Protein
Product Line
MembranePro™
For Use With (Application)
Membrane Protein Expression
Promoter
EF-1α

Contents & Storage

The kit contains:
•pEF6 V5-His TOPO™ TA Vector Kit for at least 10 TOPO™ cloning reactions (Store at -20°C)
•3 x10^6 293FT Cells (Store in liquid nitrogen)
•1 x 75 ml MembranePro™ Precipitation Mix (Store at room temperature)
•1 x 0.3 mg (1 mg⁄ml) MembranePro™ Reagent (Store at -20°C)
•3 x 0.75 ml Lipofectamine™ 2000 (Store at +4°C)
•Manuals

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