Gibco™
TrypLE™ Select Enzyme (10X), no phenol red
Description
TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A549, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:
• Gentle on cells—maintains cell health for reproducible results
• Room-temperature stable—convenient to use
• Easy to use—substitutes directly into existing protocols, with no inactivation required
• Animal origin free (AOF)—pure, consistent, and effective
This TrypLE™ Select is modified as follows:
| With | Without |
• EDTA | • Phenol Red |
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Specifications
| Chelators: | EDTA |
|---|---|
| Classification: | Animal Origin-Free |
| Enzyme Stability: | Room Temperature Stable |
| Form: | Liquid |
| Inorganic Salts: | No Calcium, No Magnesium, No Sodium Phosphate |
| Osmolality: | 270 - 320 mOsm/kg |
| Phenol Red Indicator: | No Phenol Red |
| Product Line: | Gibco® |
| Purity or Quality Grade: | Cell Culture Grade |
| Reagent Type: | Trypsin Replacement |
| Shipping Condition: | Room Temperature |
| Tested For: | In Vitro Bioassay |
| pH Range: | 6.9 - 7.4 |
| Concentration: | 10 X |
| Quantity: | 100mL |
Contents & storage
Storage conditions: 15–30°C.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.
