TrypLE™ Select Enzyme (10X), no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: A1217703

Gibco™  Related applications: Mammalian Cell Culture

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Description

TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. We offer a variety of TrypLE™ formulations for a range of cell culture applications.

Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:

Gentle on cells—maintains cell health for reproducible results
Room-temperature stable—convenient to use
Easy to use—substitutes directly into existing protocols, with no inactivation required
Animal origin free (AOF)—pure, consistent, and effective

This TrypLE™ Select is modified as follows:

WithWithout

• EDTA

• Phenol Red

The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.

Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.

Room-temperature stable
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.

Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.

Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.

Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.

Specifications

Chelators: EDTA
Phenol Red Indicator: No Phenol Red
Classification: Animal Origin-Free
Enzyme Stability: Room Temperature Stable
Form: Liquid
pH Range: 6.9 - 7.4
Osmolality: 270 - 320 mOsm/kg
Concentrated: 10 X
Product Size: 20 x 100 mL
Reagent Type: Trypsin Replacement
Inorganic Salts: No Calcium, No Magnesium, No Sodium Phosphate
Tests Performed: In Vitro Bioassay
Purity or Quality Grade: Cell Culture Grade
Green Features: Energy efficient, Less hazardous, Sustainable packaging
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 15–30°C. Protect from light.
Alternate storage conditions: 2–8°C or -5 to -20°C.
Shipping conditions: Ambient.
Shelf life: 24 months from date of manufacture.

Documents

Manuals & protocols

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