Amplex™ Red Phospholipase D Assay Kit

Catalog number: A12219

Amplex™ Red Phospholipase D Assay Kit

Catalog number: A12219
Catalog Number
Unit Size
500 assays
Price (USD)
Your Price:
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
A12219500 assays
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Product Overview
Citations & References
Additional Information
The Amplex® Red Phospholipase D Assay Kit provides a sensitive and simplemethod to detect Phospholipase D (PLD) activity using a fluorescence microplate reader or fluorometer.

See our complete line of Fluorescence Microplate assays.

• Detect phospholipase D activity levels as low as 10 U/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

PLD activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex® Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. PLD converts the phosphatidylcholine (lecithin) to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, huydrogen peroxide reacts with the Amplex® Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex® Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex® Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex® UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex® Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex® Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.


Detection Method
Fluorescence Intensity
Product Line
Shipping Condition
Room Temperature
Substrate Properties
Lipid-Based Substrate, Chemical Substrate
Substrate Type
Phospholipase Substrate
Target Enzyme
Product Type
Phospholipase Assay

Contents & Storage

Store in freezer -5°C to -30°C and protect from light.


Frequently asked questions (FAQs)

Citations & References