Alexa Fluor™ 488 Phalloidin
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Description
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye.• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 495/518 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
Get Superior Results in Your Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability.
Use in Multiple Applications
Alexa Fluor® 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 488 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot® nanocrystals and other Alexa Fluor® conjugates including secondary antibodies.
Read about labeling F-Actin with phallotoxins
Find out more about our Alexa Fluor® 488 products
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool
See the full line of Alexa Fluor® Dye products
Figures
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)
Muntjac fibroblast labeled with probes for actin and the nucleus.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein and DAPI.
FluoCells prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast.
Cultured axolotl sensory neurons. Anti–human neuronal protein HuC/HuD antibody, Alexa Fluor® 546 goat anti–mouse IgG antibody, Alexa Fluor® 488 phalloidin and Hoechst 33342.
Photobleaching comparison of fluorescein phalloidin and Alexa Fluor® 488 phalloidin.
Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX® Nuclear Labeling Kit and Alexa Fluor® phalloidin conjugates.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein phalloidin and Alexa Fluor® 546 goat anti–mouse IgG antibody.
Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.
Bovine pulmonary artery endothelial cells stained with Alexa Fluor® 488 phalloidin and DAPI.
Actin filaments of the turbellarian flatworm Archimonotresis sp.
Muntjac skin fibroblasts stained with Alexa Fluor 488 phalloidin, an anti–a-tubulin antibody and the anti–cdc6 peptide antibody.
Specifications
| Label or Dye: | Alexa Fluor™ 488 |
|---|---|
| Sub-Cellular Localization: | Actin, Cytoskeleton |
| Excitation⁄Emission (nm): | 495⁄518 |
| Product Line: | Alexa Fluor™ |
| Product Size: | 300 units |
| Shipping Condition: | Room Temperature |
