Description
Alexa Fluor 488® phalloidin is a high-affinity filamentous actin (F-actin) probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye.Also available as a room-temperature-stable, ready-to-use solution: ActinGreen 488 Ready Probes Reagent.
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• Selectively stains F-actin
• Outstanding fluorescence performance
• Excitation/Emission: 495/518 nm
• Superior to antibody staining
• Optimal for fixed and permeabilized samples
Get Superior Results in Your Actin Staining Studies
Phalloidin is a bicyclic peptide belonging to a family of toxins isolated from the deadly Amanita phalloides “death cap" mushroom and is commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 provides green fluorescence of unparalleled brightness and photostability.
Use in Multiple Applications
Alexa Fluor® 488 phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. Alexa Fluor® 488 phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses, including fluorescent proteins, Qdot® nanocrystals and other Alexa Fluor® conjugates including secondary antibodies.
Read about labeling F-Actin with phallotoxins
Find out more about our Alexa Fluor® 488 products
Create a Cellular Masterpiece with Alexa Fluor® Phalloidin using the Cell Paint Tool
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Figures
Muntjac fibroblast labeled with probes for actin and the nucleus.
Amplification of the GFP signal localized in mitochondria of a transfected HeLa cell.
Stained and Fixed Neonatal Human Dermal Fibroblasts
Muntjac skin fibroblasts stained with Alexa Fluor 488 phalloidin, an anti–a-tubulin antibody and the anti–cdc6 peptide antibody.
Cultured axolotl sensory neurons stained with our anti–human neuronal protein HuC/HuD antibody and detected using the red-orange–fluorescent Alexa Fluor® 546 goat anti–mouse IgG antibody.
Fixed, permeabilized bovine pulmonary artery endothelial cells visualized using components of the SelectFX® Nuclear Labeling Kit and Alexa Fluor® phalloidin conjugates.
Bovine pulmonary artery endothelial cells. Alexa Fluor® 488 phalloidin, fluorescein phalloidin and Alexa Fluor® 546 goat anti–mouse IgG antibody.
Photobleaching comparison of fluorescein phalloidin and Alexa Fluor® 488 phalloidin.
FluoCells prepared slide #6 showing a fixed, permeabilized, and labeled muntjac skin fibroblast.
Cultured axolotl sensory neurons. Anti–human neuronal protein HuC/HuD antibody, Alexa Fluor® 546 goat anti–mouse IgG antibody, Alexa Fluor® 488 phalloidin and Hoechst 33342.
Actin filaments of the turbellarian flatworm Archimonotresis sp.
Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126)
Bovine pulmonary artery endothelial cells stained with Alexa Fluor® 488 phalloidin and DAPI.
Specifications
| Excitation/Emission: | 495⁄518 |
|---|---|
| Label or Dye: | Alexa Fluor™ 488 |
| Product Line: | Alexa Fluor™ |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Actin, Cytoskeleton |
| Label Type: | Alexa Fluor Dyes |
