Gibco™
CHO CD EfficientFeed™ B AGT™ Nutrient Supplement
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Description
Gibco® CHO CD EfficientFeed™ B AGT™ nutrient supplement is a single part dry format product for fed batch addition to the culture of multiple cell lines. CHO CD EfficientFeed™ B AGT™ (Advanced Granulation Technology™) nutrient supplement is an animal origin-free, chemically defined formulation that contains no proteins, hydrolysates, or components of incompletely defined composition.The AGT™ format is designed for large scale manufacturing and ease of use. This product is also available in liquid format for small scale use and experimentation.
• Expect up to 2- to 5-fold increases in productivity
• Avoid issues associated with the use of hydrolysates and other alternatives while gaining superior performance
• Simplify scale-up by leveraging the value of AGT™ simple reconstitution
• Achieve performance comparable to our original liquid catalog product
• Ready for larger scale use with convenient packaging options (10 L and 100 L)
Which EfficientFeed™ Nutrient Supplement to Choose?
Consider the CHO CD Efficient Feed™ Kit when:
• You are using a base medium with less than 6 g/L glucose and no hydrolysate at day 0
Consider CHO CD EfficientFeed™ A supplement when:
• You are currently using CD OptiCHO™ as your base cell growth medium
Consider CHO CD EfficientFeed™ B supplement when:
• You are currently using CD CHO as your base cell growth medium
Consider CD EfficientFeed™ C AGT™ supplement when:
• You want higher productivity in GS CHO and other selected cell lines
• You are currently using CD FortiCHO™ as your base cell growth medium
• You are using a base medium with greater than 6 g/L glucose or a hydrolysate at day 0
Increased Titers
CHO CD EfficientFeed™ supplements have been shown to increase titers 2-5 fold versus batch culture, and are an excellent starting point for process development (Figures 1a & 1b). Furthermore, they outperform competitive alternatives, including hydrolysates (Figures 2, 3a, & 3b).
Simple Scale-Up and Cost Savings
The ability to scale up in a dry format provides ease of handling and storage in manufacturing and other bioprocess settings. With AGT™ (Advanced Granulation Technology™) dry format, it is possible to store and use the product even more easily and safely than traditional dry format products. CHO CD EfficientFeed™ B AGT™ nutrient supplement is offered in 10 L and 100 L packaging that allows scale-up from the 1 L catalog liquid as a seamless process, as well as allow for cost savings when purchasing production level volumes.
Comparable Performance to Liquid Supplement
Equally important as the convenience and simplicity of AGT™ (Advanced Granulation Technology™) dry format is the comparable performance to the liquid CHO CD EfficientFeed™ B (Figure 4). Both forms have performed similarly in our testing, and the AGT™ version has been used successfully as a prototype in many processes by multiple companies.
AGT™ Format Ease of Use
CHO CD EfficientFeed™ B AGT™ nutrient supplement delivers dry format scale-up convenience as well as simple reconstitution via water addition and a single pH adjustment step (see Product Profile Sheet below for complete instructions).
For research or further manufacturing use. Not for use in diagnostic procedures.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.
Figures
Figure 2 - Performance of CHO CD EfficientFeed™ supplements compared to hydrolysates.
Figure 1a - Fed-batch culture using CD OptiCHO™ as base medium; IgG CHO cell line A
Figure 5 - EfficientFeed™ B in the AGT™ format provide superior performance over competitive feeds
Figure 1b - Fed-batch culture using CD OptiCHO™ as base medium; IgG CHO cell line B
Figure 3 - Performance of CHO CD EfficientFeed™ supplements with hydrolysates.
Three reconstituted lots of CHO CD EfficientFeedâ„¢ B AGT were compared to a lot of catalog liquid product. (Cat. No. A10240-01) in a reconstituted DG44 CHO fedbatch shake flask experiment in CD CHO Medium. A rDG44 CHO un-fed batch culture in CD CHO Medium was an additional control. The results indicate that the 3 lots of reconstituted AGT and the liquid control performed equivalently.
Specifications
| Cell Line: | CHO |
|---|---|
| Classification: | Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free |
| Comments: | Chemically Defined Nutrient Feeding Supplement |
| Culture Type: | Suspension Cell Culture |
| Endotoxin Level: | Low |
| Form: | Powder (AGT) |
| Format: | Securitainer Capped Vial |
| Glutamine: | No Glutamine |
| Included Antibiotics: | No antibiotics |
| Other Additives: | No Insulin |
| Phenol Red Indicator: | No Phenol Red |
| Product Size: | 10 L |
| Purity or Quality Grade: | Cell Culture Grade, Qualified for Further Manufacturing, Research Grade |
| Serum Level: | Serum-Free |
| Sodium Bicarbonate Buffer: | Sodium Bicarbonate |
| Sodium Pyruvate Additive: | Sodium Pyruvate |
| Sterility: | Nonsterile |
| Tests Performed: | Solubility |
| Shipping Condition: | Wet Ice |
Contents & storage
Dry format medium
Store at 2 to 8°C, Store Dark and Dry
Store at 2 to 8°C, Store Dark and Dry
